Phenotypic diversity of hydrogen production in chlorophycean algae reflects distinct anaerobic metabolisms

Jonathan E. Meuser, Gennady Ananyev, Lauren E. Wittig, Sergey Kosourov, Maria L. Ghirardi, Michael Seibert, G Charles Dismukes, Matthew C. Posewitz

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Several species of green algae use [FeFe]-hydrogenases to oxidize and/or produce H2 during anoxia. To further define unique aspects of algal hydrogenase activity, the well-studied anaerobic metabolisms of Chlamydomonas reinhardtii were compared with four strains of Chlamydomonas moewusii and a Lobochlamys culleus strain. In vivo and in vitro hydrogenase activity, starch accumulation/degradation, and anaerobic end product secretion were analyzed. The C. moewusii strains showed the most rapid induction of hydrogenase activity, congruent with high rates of starch catabolism, and anoxic metabolite accumulation. Intriguingly, we observed significant differences in morphology and hydrogenase activity in the C. moewusii strains examined, likely the result of long-term adaptation and/or genetic drift during culture maintenance. Of the C. moewusii strains examined, SAG 24.91 showed the highest in vitro hydrogenase activity. However, SAG 24.91 produced little H2 under conditions of sulfur limitation, which is likely a consequence of its inability to utilize exogenous acetate. In L. culleus, hydrogenase activity was minimal unless pulsed light was used to induce significant H2 photoproduction. Overall, our results demonstrate that unique anaerobic acclimation strategies have evolved in distinct green algae, resulting in differential levels of hydrogenase activity and species-specific patterns of NADH reoxidation during anoxia.

Original languageEnglish
Pages (from-to)21-30
Number of pages10
JournalJournal of Biotechnology
Volume142
Issue number1
DOIs
Publication statusPublished - Jun 1 2009

Fingerprint

Anaerobiosis
Hydrogenase
Algae
Hydrogen production
Metabolism
Hydrogen
Starch
Chlorophyta
Metabolites
Sulfur
Chlamydomonas
Chlamydomonas reinhardtii
Genetic Drift
Degradation
Acclimatization
NAD
Acetates
Maintenance
Light

Keywords

  • Algae
  • Anoxia
  • Biofuel
  • Chlamydomonas
  • Hydrogenase
  • Photosynthesis

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Meuser, J. E., Ananyev, G., Wittig, L. E., Kosourov, S., Ghirardi, M. L., Seibert, M., ... Posewitz, M. C. (2009). Phenotypic diversity of hydrogen production in chlorophycean algae reflects distinct anaerobic metabolisms. Journal of Biotechnology, 142(1), 21-30. https://doi.org/10.1016/j.jbiotec.2009.01.015

Phenotypic diversity of hydrogen production in chlorophycean algae reflects distinct anaerobic metabolisms. / Meuser, Jonathan E.; Ananyev, Gennady; Wittig, Lauren E.; Kosourov, Sergey; Ghirardi, Maria L.; Seibert, Michael; Dismukes, G Charles; Posewitz, Matthew C.

In: Journal of Biotechnology, Vol. 142, No. 1, 01.06.2009, p. 21-30.

Research output: Contribution to journalArticle

Meuser, JE, Ananyev, G, Wittig, LE, Kosourov, S, Ghirardi, ML, Seibert, M, Dismukes, GC & Posewitz, MC 2009, 'Phenotypic diversity of hydrogen production in chlorophycean algae reflects distinct anaerobic metabolisms', Journal of Biotechnology, vol. 142, no. 1, pp. 21-30. https://doi.org/10.1016/j.jbiotec.2009.01.015
Meuser, Jonathan E. ; Ananyev, Gennady ; Wittig, Lauren E. ; Kosourov, Sergey ; Ghirardi, Maria L. ; Seibert, Michael ; Dismukes, G Charles ; Posewitz, Matthew C. / Phenotypic diversity of hydrogen production in chlorophycean algae reflects distinct anaerobic metabolisms. In: Journal of Biotechnology. 2009 ; Vol. 142, No. 1. pp. 21-30.
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