Photoassembly of the manganese cluster and oxygen evolution from monomeric and dimeric CP47 reaction center photosystem II complexes

Claudia Büchel, Jim Barber, Gennady Ananyev, Said Eshaghi, Richard Watt, G Charles Dismukes

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Abstract

Isolated subcomplexes of photosystem II from spinach (CP47RC), composed of D1, D2, cytochrome b559, CP47, and a number of hydrophobic small subunits but devoid of CP43 and the extrinsic proteins of the oxygen-evolving complex, were shown to reconstitute the Mn4Ca1Cl(x) cluster of the water- splitting system and to evolve oxygen. The photoactivation process in CP47RC dimers proceeds by the same two-step mechanism as observed in PSII membranes and exhibits the same stoichiometry for Mn2+, but with a 10-fold lower affinity for Ca2+ and an increased susceptibility to photodamage. After the lower Ca2+ affinity and the 10-fold smaller absorption cross-section for photons in CP47 dimers is taken into account, the intrinsic rate constant for the rate-limiting calcium-dependent dark step is indistinguishable for the two systems. The monomeric form of CP47RC also showed capacity to photoactivate and catalyze water oxidation, but with lower activity than the dimeric form and increased susceptibility to photodamage. After optimization of the various parameters affecting the photoactivation process in dimeric CP47RC subcores, 18% of the complexes were functionally reconstituted and the quantum efficiency for oxygen production by reactivated centers approached 96% of that observed for reconstituted photosystem II-enriched membranes.

Original languageEnglish
Pages (from-to)14288-14293
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume96
Issue number25
DOIs
Publication statusPublished - Dec 7 1999

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Photosystem II Protein Complex
Manganese
Oxygen
Cytochromes a
Spinacia oleracea
Membranes
Water
Photons
Calcium
Proteins

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Photoassembly of the manganese cluster and oxygen evolution from monomeric and dimeric CP47 reaction center photosystem II complexes. / Büchel, Claudia; Barber, Jim; Ananyev, Gennady; Eshaghi, Said; Watt, Richard; Dismukes, G Charles.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, No. 25, 07.12.1999, p. 14288-14293.

Research output: Contribution to journalArticle

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