Photodynamic control of bioactivity in a nanofiber matrix

Shantanu Sur, John B. Matson, Matthew J. Webber, Christina J. Newcomb, Samuel I Stupp

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

Self-assembling peptide materials have been used extensively to mimic natural extracellular matrices (ECMs) by presenting bioactive epitopes on a synthetic matrix. Although this approach can facilitate a desired response from cells grown in the matrix, it lacks the capacity for spatial or temporal regulation of the presented signals. We describe here a photoresponsive, synthetic ECM using a supramolecular platform composed of peptide amphiphiles (PAs) that self-assemble into cylindrical nanofibers. A photocleavable nitrobenzyl ester group was included in the peptide backbone using a novel Fmoc-amino acid that is compatible with microwave-assisted solid-phase peptide synthesis. The placement of the photolabile group on the peptide backbone enabled efficient removal of the ECM-derived cell adhesion epitope RGDS from PA molecules upon exposure to light (half-life of photolysis ∼1.9 min) without affecting the nanofiber assembly. Fibroblasts cultured on RGDS-presenting PA nanofiber substrates demonstrated increased cell spreading and more mature focal adhesions compared with unfunctionalized and control (RGES-presenting) surfaces, as determined by immunostaining and cell morphological analysis. Furthermore, we observed an arrest in fibroblast spreading on substrates containing a cleavable RGDS epitope when the culture was exposed to light; in contrast, this dynamic shift in cell response was absent when the RGDS epitope was attached to the PA molecule by a light-insensitive control linker. Light-responsive bioactive materials can contribute to the development of synthetic systems that more closely mimic the dynamic nature of native ECM.

Original languageEnglish
Pages (from-to)10776-10785
Number of pages10
JournalACS Nano
Volume6
Issue number12
DOIs
Publication statusPublished - Dec 21 2012

Fingerprint

Nanofibers
Bioactivity
Peptides
peptides
Amphiphiles
Epitopes
matrices
Fibroblasts
fibroblasts
activity (biology)
cells
arginyl-glycyl-aspartyl-serine
adhesion
Molecules
Cell adhesion
Photolysis
Substrates
assembling
half life
Esters

Keywords

  • cell morphological analysis
  • nitrobenzyl
  • photodegradable
  • projected cell area
  • RGDS epitope
  • supramolecular nanofibers

ASJC Scopus subject areas

  • Engineering(all)
  • Materials Science(all)
  • Physics and Astronomy(all)

Cite this

Sur, S., Matson, J. B., Webber, M. J., Newcomb, C. J., & Stupp, S. I. (2012). Photodynamic control of bioactivity in a nanofiber matrix. ACS Nano, 6(12), 10776-10785. https://doi.org/10.1021/nn304101x

Photodynamic control of bioactivity in a nanofiber matrix. / Sur, Shantanu; Matson, John B.; Webber, Matthew J.; Newcomb, Christina J.; Stupp, Samuel I.

In: ACS Nano, Vol. 6, No. 12, 21.12.2012, p. 10776-10785.

Research output: Contribution to journalArticle

Sur, S, Matson, JB, Webber, MJ, Newcomb, CJ & Stupp, SI 2012, 'Photodynamic control of bioactivity in a nanofiber matrix', ACS Nano, vol. 6, no. 12, pp. 10776-10785. https://doi.org/10.1021/nn304101x
Sur S, Matson JB, Webber MJ, Newcomb CJ, Stupp SI. Photodynamic control of bioactivity in a nanofiber matrix. ACS Nano. 2012 Dec 21;6(12):10776-10785. https://doi.org/10.1021/nn304101x
Sur, Shantanu ; Matson, John B. ; Webber, Matthew J. ; Newcomb, Christina J. ; Stupp, Samuel I. / Photodynamic control of bioactivity in a nanofiber matrix. In: ACS Nano. 2012 ; Vol. 6, No. 12. pp. 10776-10785.
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