Photoinduced reduction of the medial FeS center in the hydrogenase small subunit HupS from Nostoc punctiforme

Patrícia Raleiras, Leif Hammarström, Peter Lindblad, Stenbjörn Styring, Ann Magnuson

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The small subunit from the NiFe uptake hydrogenase, HupSL, in the cyanobacterium Nostoc punctiforme ATCC 29133, has been isolated in the absence of the large subunit (P. Raleiras, P. Kellers, P. Lindblad, S. Styring, A. Magnuson, J. Biol. Chem. 288 (2013) 18,345-18,352). Here, we have used flash photolysis to reduce the iron-sulfur clusters in the isolated small subunit, HupS. We used ascorbate as electron donor to the photogenerated excited state of Ru(II)-trisbipyridine (Ru(bpy)3), to generate Ru(I)(bpy)3 as reducing agent. Our results show that the isolated small subunit can be reduced by the Ru(I)(bpy)3 generated through flash photolysis.

Original languageEnglish
Pages (from-to)57-61
Number of pages5
JournalJournal of Inorganic Biochemistry
Publication statusPublished - Jul 2015



  • Electron paramagnetic resonance
  • Electron transfer
  • Flash photolysis
  • Hydrogenase
  • Iron-sulfur

ASJC Scopus subject areas

  • Biochemistry
  • Inorganic Chemistry

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