Photosystem I of Chlamydomonas reinhardtii contains nine light-harvesting complexes (Lhca) located on one side of the core

Bartlomiej Drop, Mariam Webber-Birungi, Fabrizia Fusetti, Roman Kourǐl, Kevin Edward Redding, Egbert J. Boekema, Roberta Croce

Research output: Contribution to journalArticle

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Abstract

In this work we have purified the Photosystem I (PSI) complex of Chlamydomonas reinhardtii to homogeneity. Biochemical, proteomic, spectroscopic, and structural analyses reveal the main properties of this PSI-LHCI supercomplex. The data show that the largest purified complex is composed of one core complex and nine Lhca antennas and that it contains all Lhca gene products. A projection map at 15 Å resolution obtained by electron microscopy reveals that the Lhcas are organized on one side of the core in a double half-ring arrangement, in contrast with previous suggestions. A series of stable disassembled PSI-LHCI intermediates was purified. The analysis of these complexes suggests the sequence of the assembly/disassembly process. It is shown that PSI-LHCI of C. reinhardtii is larger but far less stable than the complex from higher plants. Lhca2 and Lhca9 (the red-most antenna complexes), although present in the largest complex in 1:1 ratio with the core, are only loosely associated with it. This can explain the large variation in antenna composition of PSI-LHCI from C. reinhardtii found in the literature. The analysis of several subcomplexes with reduced antenna size allows determination of the position of Lhca2 and Lhca9 and leads to a proposal for a model of the organization of the Lhcas within the PSI-LHCI supercomplex.

Original languageEnglish
Pages (from-to)44878-44887
Number of pages10
JournalJournal of Biological Chemistry
Volume286
Issue number52
DOIs
Publication statusPublished - Dec 30 2011

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Photosystem I Protein Complex
Chlamydomonas reinhardtii
Light
Antennas
Size determination
Proteomics
Electron microscopy
Electron Microscopy
Genes
Chemical analysis

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

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Photosystem I of Chlamydomonas reinhardtii contains nine light-harvesting complexes (Lhca) located on one side of the core. / Drop, Bartlomiej; Webber-Birungi, Mariam; Fusetti, Fabrizia; Kourǐl, Roman; Redding, Kevin Edward; Boekema, Egbert J.; Croce, Roberta.

In: Journal of Biological Chemistry, Vol. 286, No. 52, 30.12.2011, p. 44878-44887.

Research output: Contribution to journalArticle

Drop, Bartlomiej ; Webber-Birungi, Mariam ; Fusetti, Fabrizia ; Kourǐl, Roman ; Redding, Kevin Edward ; Boekema, Egbert J. ; Croce, Roberta. / Photosystem I of Chlamydomonas reinhardtii contains nine light-harvesting complexes (Lhca) located on one side of the core. In: Journal of Biological Chemistry. 2011 ; Vol. 286, No. 52. pp. 44878-44887.
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