Photosystem II single crystals studied by EPR spectroscopy at 94 GHz

The tyrosine radical Yd•

W. Hofbauer, A. Zouni, R. Bittl, J. Kern, P. Orth, F. Lendzian, Petra Fromme, H. T. Witt, W. Lubitz

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

Electron paramagnetic resonance (EPR) spectroscopy at 94 GHz is used to study the dark-stable tyrosine radical YD in single crystals of photosystem II core complexes (cc) isolated from the thermophilic cyanobacterium Synechococcus elongatus. These complexes contain at least 17 subunits, including the water-oxidizing complex (WOC), and 32 chlorophyll a molecules/PS II; they are active in light-induced electron transfer and water oxidation. The crystals belong to the orthorhombic space group P212121, with four PS II dimers per unit cell. High-frequency EPR is used for enhancing the sensitivity of experiments performed on small single crystals as well as for increasing the spectral resolution of the g tensor components and of the different crystal sites. Magnitude and orientation of the g tensor of YD and related information on several proton hyperfine tensors are deduced from analysis of angular-dependent EPR spectra. The precise orientation of tyrosine YD in PS II is obtained as a first step in the EPR characterization of paramagnetic species in these single crystals.

Original languageEnglish
Pages (from-to)6623-6628
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume98
Issue number12
DOIs
Publication statusPublished - Jun 5 2001

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Photosystem II Protein Complex
Electron Spin Resonance Spectroscopy
Spectrum Analysis
Synechococcus
Water
Cyanobacteria
Tyrosine
Protons
Electrons
Light
tyrosine radical

ASJC Scopus subject areas

  • Genetics
  • General

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Photosystem II single crystals studied by EPR spectroscopy at 94 GHz : The tyrosine radical Yd•. / Hofbauer, W.; Zouni, A.; Bittl, R.; Kern, J.; Orth, P.; Lendzian, F.; Fromme, Petra; Witt, H. T.; Lubitz, W.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, No. 12, 05.06.2001, p. 6623-6628.

Research output: Contribution to journalArticle

Hofbauer, W. ; Zouni, A. ; Bittl, R. ; Kern, J. ; Orth, P. ; Lendzian, F. ; Fromme, Petra ; Witt, H. T. ; Lubitz, W. / Photosystem II single crystals studied by EPR spectroscopy at 94 GHz : The tyrosine radical Yd•. In: Proceedings of the National Academy of Sciences of the United States of America. 2001 ; Vol. 98, No. 12. pp. 6623-6628.
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AU - Kern, J.

AU - Orth, P.

AU - Lendzian, F.

AU - Fromme, Petra

AU - Witt, H. T.

AU - Lubitz, W.

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