Photosystem II single crystals studied by EPR spectroscopy at 94 GHz: The tyrosine radical Yd•

W. Hofbauer, A. Zouni, R. Bittl, J. Kern, P. Orth, F. Lendzian, Petra Fromme, H. T. Witt, W. Lubitz

Research output: Contribution to journalArticle

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Abstract

Electron paramagnetic resonance (EPR) spectroscopy at 94 GHz is used to study the dark-stable tyrosine radical YD in single crystals of photosystem II core complexes (cc) isolated from the thermophilic cyanobacterium Synechococcus elongatus. These complexes contain at least 17 subunits, including the water-oxidizing complex (WOC), and 32 chlorophyll a molecules/PS II; they are active in light-induced electron transfer and water oxidation. The crystals belong to the orthorhombic space group P212121, with four PS II dimers per unit cell. High-frequency EPR is used for enhancing the sensitivity of experiments performed on small single crystals as well as for increasing the spectral resolution of the g tensor components and of the different crystal sites. Magnitude and orientation of the g tensor of YD and related information on several proton hyperfine tensors are deduced from analysis of angular-dependent EPR spectra. The precise orientation of tyrosine YD in PS II is obtained as a first step in the EPR characterization of paramagnetic species in these single crystals.

Original languageEnglish
Pages (from-to)6623-6628
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume98
Issue number12
DOIs
Publication statusPublished - Jun 5 2001

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Photosystem II Protein Complex
Electron Spin Resonance Spectroscopy
Spectrum Analysis
Synechococcus
Water
Cyanobacteria
Tyrosine
Protons
Electrons
Light
tyrosine radical

ASJC Scopus subject areas

  • Genetics
  • General

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Photosystem II single crystals studied by EPR spectroscopy at 94 GHz : The tyrosine radical Yd•. / Hofbauer, W.; Zouni, A.; Bittl, R.; Kern, J.; Orth, P.; Lendzian, F.; Fromme, Petra; Witt, H. T.; Lubitz, W.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, No. 12, 05.06.2001, p. 6623-6628.

Research output: Contribution to journalArticle

Hofbauer, W. ; Zouni, A. ; Bittl, R. ; Kern, J. ; Orth, P. ; Lendzian, F. ; Fromme, Petra ; Witt, H. T. ; Lubitz, W. / Photosystem II single crystals studied by EPR spectroscopy at 94 GHz : The tyrosine radical Yd•. In: Proceedings of the National Academy of Sciences of the United States of America. 2001 ; Vol. 98, No. 12. pp. 6623-6628.
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AU - Kern, J.

AU - Orth, P.

AU - Lendzian, F.

AU - Fromme, Petra

AU - Witt, H. T.

AU - Lubitz, W.

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