Abstract
Bacteriorhodopsin (BR) has become recognized as an important system for modeling proton and ion transport mechanisms across protein membranes. These membrane processes are initiated optically by visible excitation of the retinal chromophore which undergoes a cyclic series of structural and conformational changes including isomerization and deprotonation. Vibrational resonance Raman spectroscopy derived from spontaneous scattering has become a major experimental technique in measuring molecular structures and conformations, including those of retinal contained in BR. Laser techniques designed to record time-resolved resonance Raman (TR**3) spectra have made it feasible to obtain the vibrational Raman spectra of transient forms of retinal generated during the photocycle of BR and to elucidate the dynamical mechanisms which underlie transport across the BR membrane. The laser and optical methods utilized in a picosecond TR**3 spectrometer and its application to retinal intermediates formed on the picosecond time scale are described in this paper.
| Original language | English |
|---|---|
| Title of host publication | Proceedings of SPIE - The International Society for Optical Engineering |
| Editors | Mustafa A. El-Sayed |
| Publisher | SPIE |
| Pages | 82-88 |
| Number of pages | 7 |
| Volume | 620 |
| ISBN (Print) | 0892526556 |
| Publication status | Published - 1986 |
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ASJC Scopus subject areas
- Electrical and Electronic Engineering
- Condensed Matter Physics
Cite this
PICOSECOND TIME-RESOLVED RESONANCE RAMAN SPECTROSCOPY OF BACTERIORHODOPSIN INTERMEDIATES. / Atkinson, G. H.; Brack, T. L.; Grieger, I.; Rumbles, Gary; Blanchard, D.; Siemenkowski, L.
Proceedings of SPIE - The International Society for Optical Engineering. ed. / Mustafa A. El-Sayed. Vol. 620 SPIE, 1986. p. 82-88.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - PICOSECOND TIME-RESOLVED RESONANCE RAMAN SPECTROSCOPY OF BACTERIORHODOPSIN INTERMEDIATES.
AU - Atkinson, G. H.
AU - Brack, T. L.
AU - Grieger, I.
AU - Rumbles, Gary
AU - Blanchard, D.
AU - Siemenkowski, L.
PY - 1986
Y1 - 1986
N2 - Bacteriorhodopsin (BR) has become recognized as an important system for modeling proton and ion transport mechanisms across protein membranes. These membrane processes are initiated optically by visible excitation of the retinal chromophore which undergoes a cyclic series of structural and conformational changes including isomerization and deprotonation. Vibrational resonance Raman spectroscopy derived from spontaneous scattering has become a major experimental technique in measuring molecular structures and conformations, including those of retinal contained in BR. Laser techniques designed to record time-resolved resonance Raman (TR**3) spectra have made it feasible to obtain the vibrational Raman spectra of transient forms of retinal generated during the photocycle of BR and to elucidate the dynamical mechanisms which underlie transport across the BR membrane. The laser and optical methods utilized in a picosecond TR**3 spectrometer and its application to retinal intermediates formed on the picosecond time scale are described in this paper.
AB - Bacteriorhodopsin (BR) has become recognized as an important system for modeling proton and ion transport mechanisms across protein membranes. These membrane processes are initiated optically by visible excitation of the retinal chromophore which undergoes a cyclic series of structural and conformational changes including isomerization and deprotonation. Vibrational resonance Raman spectroscopy derived from spontaneous scattering has become a major experimental technique in measuring molecular structures and conformations, including those of retinal contained in BR. Laser techniques designed to record time-resolved resonance Raman (TR**3) spectra have made it feasible to obtain the vibrational Raman spectra of transient forms of retinal generated during the photocycle of BR and to elucidate the dynamical mechanisms which underlie transport across the BR membrane. The laser and optical methods utilized in a picosecond TR**3 spectrometer and its application to retinal intermediates formed on the picosecond time scale are described in this paper.
UR - http://www.scopus.com/inward/record.url?scp=0022917680&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0022917680&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:0022917680
SN - 0892526556
VL - 620
SP - 82
EP - 88
BT - Proceedings of SPIE - The International Society for Optical Engineering
A2 - El-Sayed, Mustafa A.
PB - SPIE
ER -