PICOSECOND TIME-RESOLVED RESONANCE RAMAN SPECTROSCOPY OF BACTERIORHODOPSIN INTERMEDIATES.

G. H. Atkinson, T. L. Brack, I. Grieger, Gary Rumbles, D. Blanchard, L. Siemenkowski

Research output: Chapter in Book/Report/Conference proceedingConference contribution

5 Citations (Scopus)

Abstract

Bacteriorhodopsin (BR) has become recognized as an important system for modeling proton and ion transport mechanisms across protein membranes. These membrane processes are initiated optically by visible excitation of the retinal chromophore which undergoes a cyclic series of structural and conformational changes including isomerization and deprotonation. Vibrational resonance Raman spectroscopy derived from spontaneous scattering has become a major experimental technique in measuring molecular structures and conformations, including those of retinal contained in BR. Laser techniques designed to record time-resolved resonance Raman (TR**3) spectra have made it feasible to obtain the vibrational Raman spectra of transient forms of retinal generated during the photocycle of BR and to elucidate the dynamical mechanisms which underlie transport across the BR membrane. The laser and optical methods utilized in a picosecond TR**3 spectrometer and its application to retinal intermediates formed on the picosecond time scale are described in this paper.

Original languageEnglish
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
EditorsMustafa A. El-Sayed
PublisherSPIE
Pages82-88
Number of pages7
Volume620
ISBN (Print)0892526556
Publication statusPublished - 1986

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Raman spectroscopy
membranes
Membranes
Deprotonation
Lasers
Chromophores
Isomerization
vibrational spectra
Molecular structure
chromophores
isomerization
lasers
Conformations
Spectrometers
Raman scattering
Protons
molecular structure
optics
Scattering
spectrometers

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics

Cite this

Atkinson, G. H., Brack, T. L., Grieger, I., Rumbles, G., Blanchard, D., & Siemenkowski, L. (1986). PICOSECOND TIME-RESOLVED RESONANCE RAMAN SPECTROSCOPY OF BACTERIORHODOPSIN INTERMEDIATES. In M. A. El-Sayed (Ed.), Proceedings of SPIE - The International Society for Optical Engineering (Vol. 620, pp. 82-88). SPIE.

PICOSECOND TIME-RESOLVED RESONANCE RAMAN SPECTROSCOPY OF BACTERIORHODOPSIN INTERMEDIATES. / Atkinson, G. H.; Brack, T. L.; Grieger, I.; Rumbles, Gary; Blanchard, D.; Siemenkowski, L.

Proceedings of SPIE - The International Society for Optical Engineering. ed. / Mustafa A. El-Sayed. Vol. 620 SPIE, 1986. p. 82-88.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Atkinson, GH, Brack, TL, Grieger, I, Rumbles, G, Blanchard, D & Siemenkowski, L 1986, PICOSECOND TIME-RESOLVED RESONANCE RAMAN SPECTROSCOPY OF BACTERIORHODOPSIN INTERMEDIATES. in MA El-Sayed (ed.), Proceedings of SPIE - The International Society for Optical Engineering. vol. 620, SPIE, pp. 82-88.
Atkinson GH, Brack TL, Grieger I, Rumbles G, Blanchard D, Siemenkowski L. PICOSECOND TIME-RESOLVED RESONANCE RAMAN SPECTROSCOPY OF BACTERIORHODOPSIN INTERMEDIATES. In El-Sayed MA, editor, Proceedings of SPIE - The International Society for Optical Engineering. Vol. 620. SPIE. 1986. p. 82-88
Atkinson, G. H. ; Brack, T. L. ; Grieger, I. ; Rumbles, Gary ; Blanchard, D. ; Siemenkowski, L. / PICOSECOND TIME-RESOLVED RESONANCE RAMAN SPECTROSCOPY OF BACTERIORHODOPSIN INTERMEDIATES. Proceedings of SPIE - The International Society for Optical Engineering. editor / Mustafa A. El-Sayed. Vol. 620 SPIE, 1986. pp. 82-88
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