Abstract
The resonance Raman (RR) spectra in the fingerprint region (1100-1300 cm-1) are reported for the initial, picosecond interval of the bacteriorhodopsin (BR) photocycle during which the J-625 and K-590 intermediates are formed. These are the first RR features assigned to J-625 while the RR bands assignable to K-590 alone are clarified with respect to previous studies. The assignment of RR features to J-625 and K-590 is based on the results of two-laser, picosecond time-resolved resonance Raman (PTR3) experiments designed to separate RR bands of K-590 from other species. The instrumental design underlying PTR3 experiments and the associated advantages for analyzing time-resolved data are described. The PTR3 data in the fingerprint region suggest that neither J-625 nor K-590 contain all-trans retinal and therefore, establish that the primary BR photocycle event involves a configurational change in the retinal chromophore. The RR fingerprint bands assignable to J-625 and K-590, however, differ from one another indicating that the two intermediates do not contain retinal in the same configurational or conformational form and that a second change in retinal structure occurs over the initial 10 ps of the photocycle. The identification of either intermediate in terms of an absolute configuration (e.g., 13-cis retinal) or conformation remains unresolved.
| Original language | English |
|---|---|
| Pages (from-to) | 1-15 |
| Number of pages | 15 |
| Journal | Chemical Physics |
| Volume | 131 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Mar 1 1989 |
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ASJC Scopus subject areas
- Physical and Theoretical Chemistry
- Spectroscopy
- Atomic and Molecular Physics, and Optics
Cite this
Picosecond time-resolved resonance Raman spectroscopy of the initial trans to cis isomerization in the bacteriorhodopsin photocycle. / Atkinson, G. H.; Brack, T. L.; Blanchard, D.; Rumbles, Gary.
In: Chemical Physics, Vol. 131, No. 1, 01.03.1989, p. 1-15.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Picosecond time-resolved resonance Raman spectroscopy of the initial trans to cis isomerization in the bacteriorhodopsin photocycle
AU - Atkinson, G. H.
AU - Brack, T. L.
AU - Blanchard, D.
AU - Rumbles, Gary
PY - 1989/3/1
Y1 - 1989/3/1
N2 - The resonance Raman (RR) spectra in the fingerprint region (1100-1300 cm-1) are reported for the initial, picosecond interval of the bacteriorhodopsin (BR) photocycle during which the J-625 and K-590 intermediates are formed. These are the first RR features assigned to J-625 while the RR bands assignable to K-590 alone are clarified with respect to previous studies. The assignment of RR features to J-625 and K-590 is based on the results of two-laser, picosecond time-resolved resonance Raman (PTR3) experiments designed to separate RR bands of K-590 from other species. The instrumental design underlying PTR3 experiments and the associated advantages for analyzing time-resolved data are described. The PTR3 data in the fingerprint region suggest that neither J-625 nor K-590 contain all-trans retinal and therefore, establish that the primary BR photocycle event involves a configurational change in the retinal chromophore. The RR fingerprint bands assignable to J-625 and K-590, however, differ from one another indicating that the two intermediates do not contain retinal in the same configurational or conformational form and that a second change in retinal structure occurs over the initial 10 ps of the photocycle. The identification of either intermediate in terms of an absolute configuration (e.g., 13-cis retinal) or conformation remains unresolved.
AB - The resonance Raman (RR) spectra in the fingerprint region (1100-1300 cm-1) are reported for the initial, picosecond interval of the bacteriorhodopsin (BR) photocycle during which the J-625 and K-590 intermediates are formed. These are the first RR features assigned to J-625 while the RR bands assignable to K-590 alone are clarified with respect to previous studies. The assignment of RR features to J-625 and K-590 is based on the results of two-laser, picosecond time-resolved resonance Raman (PTR3) experiments designed to separate RR bands of K-590 from other species. The instrumental design underlying PTR3 experiments and the associated advantages for analyzing time-resolved data are described. The PTR3 data in the fingerprint region suggest that neither J-625 nor K-590 contain all-trans retinal and therefore, establish that the primary BR photocycle event involves a configurational change in the retinal chromophore. The RR fingerprint bands assignable to J-625 and K-590, however, differ from one another indicating that the two intermediates do not contain retinal in the same configurational or conformational form and that a second change in retinal structure occurs over the initial 10 ps of the photocycle. The identification of either intermediate in terms of an absolute configuration (e.g., 13-cis retinal) or conformation remains unresolved.
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U2 - 10.1016/0301-0104(89)87077-6
DO - 10.1016/0301-0104(89)87077-6
M3 - Article
VL - 131
SP - 1
EP - 15
JO - Chemical Physics
JF - Chemical Physics
SN - 0301-0104
IS - 1
ER -