PNA-peptide assembly in a 3D DNA nanocage at room temperature

Justin D. Flory, Sandip Shinde, Su Lin, Yan Liu, Hao Yan, Giovanna Ghirlanda, Petra Fromme

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Proteins and peptides fold into dynamic structures that access a broad functional landscape; however, designing artificial polypeptide systems is still a great challenge. Conversely, DNA engineering is now routinely used to build a wide variety of 2D and 3D nanostructures from hybridization based rules, and their functional diversity can be significantly expanded through site specific incorporation of the appropriate guest molecules. Here we demonstrate a new approach to rationally design 3D nucleic acid-amino acid complexes using peptide nucleic acid (PNA) to assemble peptides inside a 3D DNA nanocage. The PNA-peptides were found to bind to the preassembled DNA nanocage in 5-10 min at room temperature, and assembly could be performed in a stepwise fashion. Biophysical characterization of the DNA-PNA-peptide complex was performed using gel electrophoresis as well as steady state and time-resolved fluorescence spectroscopy. Based on these results we have developed a model for the arrangement of the PNA-peptides inside the DNA nanocage. This work demonstrates a flexible new approach to leverage rationally designed nucleic acid (DNA-PNA) nanoscaffolds to guide polypeptide engineering.

Original languageEnglish
Pages (from-to)6985-6993
Number of pages9
JournalJournal of the American Chemical Society
Volume135
Issue number18
DOIs
Publication statusPublished - May 8 2013

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

Fingerprint Dive into the research topics of 'PNA-peptide assembly in a 3D DNA nanocage at room temperature'. Together they form a unique fingerprint.

  • Cite this