Precision templating with DNA of a virus-like particle with peptide nanostructures

Yves Ruff, Tyson Moyer, Christina J. Newcomb, Borries Demeler, Samuel I Stupp

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

We report here the preparation of filamentous virus-like particles by the encapsulation of a linear or circular double-stranded DNA template with preassembled mushroom-shaped nanostructures having a positively charged domain. These nanostructures mimic the capsid proteins of natural filamentous viruses and are formed by self-assembly of coiled-coil peptides conjugated at opposite termini with cationic segments and poly(ethylene glycol) (PEG) chains. We found that a high molecular weight of PEG segments was critical for the formation of monodisperse and uniformly shaped filamentous complexes. It is proposed that electrostatic attachment of the nanostructures with sufficiently long PEG segments generates steric forces that increase the rigidity of the neutralized DNA template. This stiffening counterbalances the natural tendency of the DNA template to condense into toroids or buckle multiple times. The control achieved over both shape and dimensions of the particles offers a strategy to create one-dimensional supramolecular nanostructures of defined length containing nucleic acids.

Original languageEnglish
Pages (from-to)6211-6219
Number of pages9
JournalJournal of the American Chemical Society
Volume135
Issue number16
DOIs
Publication statusPublished - Apr 24 2013

Fingerprint

DNA Viruses
Nanostructures
Viruses
Virion
Peptides
Polyethylene glycols
DNA
Ethylene Glycol
Agaricales
Nucleic acids
Capsid Proteins
Static Electricity
Encapsulation
Rigidity
Self assembly
Nucleic Acids
Electrostatics
Molecular Weight
Molecular weight
Proteins

ASJC Scopus subject areas

  • Chemistry(all)
  • Catalysis
  • Biochemistry
  • Colloid and Surface Chemistry

Cite this

Precision templating with DNA of a virus-like particle with peptide nanostructures. / Ruff, Yves; Moyer, Tyson; Newcomb, Christina J.; Demeler, Borries; Stupp, Samuel I.

In: Journal of the American Chemical Society, Vol. 135, No. 16, 24.04.2013, p. 6211-6219.

Research output: Contribution to journalArticle

Ruff, Yves ; Moyer, Tyson ; Newcomb, Christina J. ; Demeler, Borries ; Stupp, Samuel I. / Precision templating with DNA of a virus-like particle with peptide nanostructures. In: Journal of the American Chemical Society. 2013 ; Vol. 135, No. 16. pp. 6211-6219.
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