Preparation of antibody-conjugated gold nanoparticles

Anthony J. Di Pasqua, Richard E. Mishler, Yan Li Ship, James C. Dabrowiak, Teddy Asefa

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

We here describe a method for the simple synthesis of gold nanoparticles (~ 10 nm in diameter) conjugated with antibody to E. coli O157:H7. Gold nanoparticles with pendant carboxylic acid and alcohol functional groups were synthesized and characterized using transmission electron microscopy (TEM) and infrared spectroscopy. These nanoparticles were then reacted with anti-E. coli O157:H7, using EDC coupling chemistry, and the product was characterized with X-ray photoelectron spectroscopy. Furthermore, binding of the antibody-gold conjugates to E. coli O157:H7 was demonstrated using transmission electron microscopy.

Original languageEnglish
Pages (from-to)1876-1879
Number of pages4
JournalMaterials Letters
Volume63
Issue number21
DOIs
Publication statusPublished - Aug 31 2009

Fingerprint

antibodies
Antibodies
Gold
Escherichia coli
gold
Nanoparticles
nanoparticles
preparation
Transmission electron microscopy
transmission electron microscopy
Carboxylic Acids
Carboxylic acids
carboxylic acids
Functional groups
Infrared spectroscopy
alcohols
Alcohols
X ray photoelectron spectroscopy
infrared spectroscopy
photoelectron spectroscopy

Keywords

  • Antibody
  • E. coli
  • Gold nanoparticles
  • Pathogen

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanical Engineering
  • Mechanics of Materials

Cite this

Di Pasqua, A. J., Mishler, R. E., Ship, Y. L., Dabrowiak, J. C., & Asefa, T. (2009). Preparation of antibody-conjugated gold nanoparticles. Materials Letters, 63(21), 1876-1879. https://doi.org/10.1016/j.matlet.2009.05.070

Preparation of antibody-conjugated gold nanoparticles. / Di Pasqua, Anthony J.; Mishler, Richard E.; Ship, Yan Li; Dabrowiak, James C.; Asefa, Teddy.

In: Materials Letters, Vol. 63, No. 21, 31.08.2009, p. 1876-1879.

Research output: Contribution to journalArticle

Di Pasqua, AJ, Mishler, RE, Ship, YL, Dabrowiak, JC & Asefa, T 2009, 'Preparation of antibody-conjugated gold nanoparticles', Materials Letters, vol. 63, no. 21, pp. 1876-1879. https://doi.org/10.1016/j.matlet.2009.05.070
Di Pasqua AJ, Mishler RE, Ship YL, Dabrowiak JC, Asefa T. Preparation of antibody-conjugated gold nanoparticles. Materials Letters. 2009 Aug 31;63(21):1876-1879. https://doi.org/10.1016/j.matlet.2009.05.070
Di Pasqua, Anthony J. ; Mishler, Richard E. ; Ship, Yan Li ; Dabrowiak, James C. ; Asefa, Teddy. / Preparation of antibody-conjugated gold nanoparticles. In: Materials Letters. 2009 ; Vol. 63, No. 21. pp. 1876-1879.
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