Preparation of antibody-conjugated gold nanoparticles

Anthony J. Di Pasqua, Richard E. Mishler, Yan Li Ship, James C. Dabrowiak, Tewodros Asefa

Research output: Contribution to journalArticlepeer-review

44 Citations (Scopus)

Abstract

We here describe a method for the simple synthesis of gold nanoparticles (~ 10 nm in diameter) conjugated with antibody to E. coli O157:H7. Gold nanoparticles with pendant carboxylic acid and alcohol functional groups were synthesized and characterized using transmission electron microscopy (TEM) and infrared spectroscopy. These nanoparticles were then reacted with anti-E. coli O157:H7, using EDC coupling chemistry, and the product was characterized with X-ray photoelectron spectroscopy. Furthermore, binding of the antibody-gold conjugates to E. coli O157:H7 was demonstrated using transmission electron microscopy.

Original languageEnglish
Pages (from-to)1876-1879
Number of pages4
JournalMaterials Letters
Volume63
Issue number21
DOIs
Publication statusPublished - Aug 31 2009

Keywords

  • Antibody
  • E. coli
  • Gold nanoparticles
  • Pathogen

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanics of Materials
  • Mechanical Engineering

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