Presentation and recognition of biotin on nanofibers formed by branched peptide amphiphiles

Mustafa O. Guler, Stephen Soukasene, James F. Hulvat, Samuel I. Stupp

Research output: Contribution to journalArticle

102 Citations (Scopus)

Abstract

A branched peptide amphiphile system was designed for enhanced recognition of biotin on nanofibers formed by self-assembly of these molecules. Branching at a lysine residue was used to design peptide amphiphiles that are capable of presenting more than one epitope per molecule. We found that biotinylated branched structures form nanofibers that enhance recognition by the avidin protein receptor relative to similar nanostructures formed by linear peptide analogues. Biotin-avidin binding to the supramolecular nanofibers was characterized by measurement of fluorescence from nanofibers incubated with chropmophore-conjugated avidin.

Original languageEnglish
Pages (from-to)249-252
Number of pages4
JournalNano letters
Volume5
Issue number2
DOIs
Publication statusPublished - Feb 1 2005

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ASJC Scopus subject areas

  • Bioengineering
  • Chemistry(all)
  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanical Engineering

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