Presentation and recognition of biotin on nanofibers formed by branched peptide amphiphiles

Mustafa O. Guler, Stephen Soukasene, James F. Hulvat, Samuel I Stupp

Research output: Contribution to journalArticle

100 Citations (Scopus)

Abstract

A branched peptide amphiphile system was designed for enhanced recognition of biotin on nanofibers formed by self-assembly of these molecules. Branching at a lysine residue was used to design peptide amphiphiles that are capable of presenting more than one epitope per molecule. We found that biotinylated branched structures form nanofibers that enhance recognition by the avidin protein receptor relative to similar nanostructures formed by linear peptide analogues. Biotin-avidin binding to the supramolecular nanofibers was characterized by measurement of fluorescence from nanofibers incubated with chropmophore-conjugated avidin.

Original languageEnglish
Pages (from-to)249-252
Number of pages4
JournalNano Letters
Volume5
Issue number2
DOIs
Publication statusPublished - Feb 2005

Fingerprint

Amphiphiles
biotin
Biotin
Nanofibers
Peptides
peptides
Avidin
lysine
Epitopes
Molecules
self assembly
molecules
Self assembly
analogs
Lysine
proteins
Nanostructures
fluorescence
Fluorescence
Proteins

ASJC Scopus subject areas

  • Materials Science(all)
  • Electronic, Optical and Magnetic Materials
  • Chemistry (miscellaneous)

Cite this

Presentation and recognition of biotin on nanofibers formed by branched peptide amphiphiles. / Guler, Mustafa O.; Soukasene, Stephen; Hulvat, James F.; Stupp, Samuel I.

In: Nano Letters, Vol. 5, No. 2, 02.2005, p. 249-252.

Research output: Contribution to journalArticle

Guler, Mustafa O. ; Soukasene, Stephen ; Hulvat, James F. ; Stupp, Samuel I. / Presentation and recognition of biotin on nanofibers formed by branched peptide amphiphiles. In: Nano Letters. 2005 ; Vol. 5, No. 2. pp. 249-252.
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