@article{d89a43ae479b408f9c4cbf6da93d9ebf,
title = "Proapoptotic Peptide Brush Polymer Nanoparticles via Photoinitiated Polymerization-Induced Self-Assembly",
abstract = "Herein, we report the photoinitiated polymerization-induced self-assembly (photo-PISA) of spherical micelles consisting of proapoptotic peptide–polymer amphiphiles. The one-pot synthetic approach yielded micellar nanoparticles at high concentrations and at scale (150 mg mL−1) with tunable peptide loadings up to 48 wt. %. The size of the micellar nanoparticles was tuned by varying the lengths of hydrophobic and hydrophilic building blocks. Critically, the peptide-functionalized nanoparticles imbued the proapoptotic “KLA” peptides (amino acid sequence: KLAKLAKKLAKLAK) with two key properties otherwise not inherent to the sequence: 1) proteolytic resistance compared to the oligopeptide alone; 2) significantly enhanced cell uptake by multivalent display of KLA peptide brushes. The result was demonstrated improved apoptosis efficiency in HeLa cells. These results highlight the potential of photo-PISA in the large-scale synthesis of functional, proteolytically resistant peptide–polymer conjugates for intracellular delivery.",
keywords = "nanoparticles, peptide delivery, peptide–polymer conjugates, polymerization, scaled synthesis",
author = "Hao Sun and Wei Cao and Nanzhi Zang and Clemons, {Tristan D.} and Scheutz, {Georg M.} and Ziying Hu and Thompson, {Matthew P.} and Yifei Liang and Maria Vratsanos and Xuhao Zhou and Wonmin Choi and Sumerlin, {Brent S.} and Stupp, {Samuel I.} and Gianneschi, {Nathan C.}",
note = "Funding Information: The authors are grateful for the support of the NIH through the NHLBI (R01HL139001), National Science Foundation fund grants (DMR‐1710105 and DMR‐1904631), and an AFOSR MURI (FA9550‐16‐0150). Additional support was provided by the Center for Regenerative Nanomedicine in the Simpson Querrey Institute at Northwestern University. TDC acknowledges the support from an American Australian Association Dow Chemical Company Fellowship. Funding Information: The authors are grateful for the support of the NIH through the NHLBI (R01HL139001), National Science Foundation fund grants (DMR-1710105 and DMR-1904631), and an AFOSR MURI (FA9550-16-0150). Additional support was provided by the Center for Regenerative Nanomedicine in the Simpson Querrey Institute at Northwestern University. TDC acknowledges the support from an American Australian Association Dow Chemical Company Fellowship. Publisher Copyright: {\textcopyright} 2020 Wiley-VCH GmbH",
year = "2020",
month = oct,
day = "19",
doi = "10.1002/anie.202006385",
language = "English",
volume = "59",
pages = "19136--19142",
journal = "Angewandte Chemie - International Edition",
issn = "1433-7851",
publisher = "John Wiley and Sons Ltd",
number = "43",
}