Programming molecular topologies from single-stranded nucleic acids

Xiaodong Qi, Fei Zhang, Zhaoming Su, Shuoxing Jiang, Dongran Han, Baoquan Ding, Yan Liu, Wah Chiu, Peng Yin, Hao Yan

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9 Citations (Scopus)

Abstract

Molecular knots represent one of the most extraordinary topological structures in biological polymers. Creating highly knotted nanostructures with well-defined and sophisticated geometries and topologies remains challenging. Here, we demonstrate a general strategy to design and construct highly knotted nucleic acid nanostructures, each weaved from a single-stranded DNA or RNA chain by hierarchical folding in a prescribed order. Sets of DNA and RNA knots of two- or three-dimensional shapes have been designed and constructed (ranging from 1700 to 7500 nucleotides), and they exhibit complex topological features, with high crossing numbers (from 9 up to 57). These single-stranded DNA/RNA knots can be replicated and amplified enzymatically in vitro and in vivo. This work establishes a general platform for constructing nucleic acid nanostructures with complex molecular topologies.

Original languageEnglish
Article number4579
JournalNature communications
Volume9
Issue number1
DOIs
Publication statusPublished - Dec 1 2018

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ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

Qi, X., Zhang, F., Su, Z., Jiang, S., Han, D., Ding, B., Liu, Y., Chiu, W., Yin, P., & Yan, H. (2018). Programming molecular topologies from single-stranded nucleic acids. Nature communications, 9(1), [4579]. https://doi.org/10.1038/s41467-018-07039-7