Programming molecular topologies from single-stranded nucleic acids

Xiaodong Qi, Fei Zhang, Zhaoming Su, Shuoxing Jiang, Dongran Han, Baoquan Ding, Yan Liu, Wah Chiu, Peng Yin, Hao Yan

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Molecular knots represent one of the most extraordinary topological structures in biological polymers. Creating highly knotted nanostructures with well-defined and sophisticated geometries and topologies remains challenging. Here, we demonstrate a general strategy to design and construct highly knotted nucleic acid nanostructures, each weaved from a single-stranded DNA or RNA chain by hierarchical folding in a prescribed order. Sets of DNA and RNA knots of two- or three-dimensional shapes have been designed and constructed (ranging from 1700 to 7500 nucleotides), and they exhibit complex topological features, with high crossing numbers (from 9 up to 57). These single-stranded DNA/RNA knots can be replicated and amplified enzymatically in vitro and in vivo. This work establishes a general platform for constructing nucleic acid nanostructures with complex molecular topologies.

Original languageEnglish
Article number4579
JournalNature Communications
Volume9
Issue number1
DOIs
Publication statusPublished - Dec 1 2018

Fingerprint

Nanostructures
nucleic acids
programming
Nucleic Acids
topology
deoxyribonucleic acid
Single-Stranded DNA
Topology
RNA
nucleotides
folding
Polymers
Nucleotides
platforms
Geometry
DNA
polymers
geometry

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

Programming molecular topologies from single-stranded nucleic acids. / Qi, Xiaodong; Zhang, Fei; Su, Zhaoming; Jiang, Shuoxing; Han, Dongran; Ding, Baoquan; Liu, Yan; Chiu, Wah; Yin, Peng; Yan, Hao.

In: Nature Communications, Vol. 9, No. 1, 4579, 01.12.2018.

Research output: Contribution to journalArticle

Qi, X, Zhang, F, Su, Z, Jiang, S, Han, D, Ding, B, Liu, Y, Chiu, W, Yin, P & Yan, H 2018, 'Programming molecular topologies from single-stranded nucleic acids', Nature Communications, vol. 9, no. 1, 4579. https://doi.org/10.1038/s41467-018-07039-7
Qi, Xiaodong ; Zhang, Fei ; Su, Zhaoming ; Jiang, Shuoxing ; Han, Dongran ; Ding, Baoquan ; Liu, Yan ; Chiu, Wah ; Yin, Peng ; Yan, Hao. / Programming molecular topologies from single-stranded nucleic acids. In: Nature Communications. 2018 ; Vol. 9, No. 1.
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