Quantitative assessment of intrinsic carbonic anhydrase activity and the capacity for bicarbonate oxidation in photosystem II

Warwick Hillier, Iain McConnell, Murray R. Badger, Alain Boussac, Vyacheslav V. Klimov, G Charles Dismukes, Tom Wydrzynski

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

On the basis of equilibrium isotopic distribution experiments using 18O-labeled water, it is generally accepted that water is the sole substrate for O2 production by photosystem II (PSII). Nevertheless, recent studies indicating a direct interaction between bicarbonate and the donor side of PSII have been used to hypothesize that bicarbonate may have been a physiologically important substrate for O2 production during the evolution of PSII [Dismukes, G. C., Klimov, V. V., Baranov, S. V., Kozlov, Y. N., DasGupta, J., and Tyryshikin, A. (2001) Proc. Natl. Acad. Sci. U.S.A. 98, 2170-2175]. To test out this hypothesis and to determine whether contemporary oxygenic organisms have the capacity to oxidize bicarbonate, we employed special rapid-mixing isotopic experiments using 18O/13C-labeled bicarbonate to quantify the inherent carbonic anhydrase activity in PSII samples and the potential flux of oxygen from bicarbonate into the photosynthetically produced O2. The measurements were made on PSII samples prepared from spinach, Thermosynechococcus elongatus, and Arthrospira maxima. For the latter organism, a strain was used that grows naturally in an alkaline, high (bi)carbonate soda lake in Africa. The results reveal that bicarbonate is not the substrate for O2 production in these contemporary oxygenic photoautotrophs when assayed under single turnover conditions.

Original languageEnglish
Pages (from-to)2094-2102
Number of pages9
JournalBiochemistry
Volume45
Issue number7
DOIs
Publication statusPublished - Feb 21 2006

Fingerprint

Carbonic Anhydrases
Photosystem II Protein Complex
Bicarbonates
Oxidation
Substrates
Spirulina
Spinacia oleracea
Water
Carbonates
Lakes
Experiments
Oxygen
Fluxes

ASJC Scopus subject areas

  • Biochemistry

Cite this

Quantitative assessment of intrinsic carbonic anhydrase activity and the capacity for bicarbonate oxidation in photosystem II. / Hillier, Warwick; McConnell, Iain; Badger, Murray R.; Boussac, Alain; Klimov, Vyacheslav V.; Dismukes, G Charles; Wydrzynski, Tom.

In: Biochemistry, Vol. 45, No. 7, 21.02.2006, p. 2094-2102.

Research output: Contribution to journalArticle

Hillier, Warwick ; McConnell, Iain ; Badger, Murray R. ; Boussac, Alain ; Klimov, Vyacheslav V. ; Dismukes, G Charles ; Wydrzynski, Tom. / Quantitative assessment of intrinsic carbonic anhydrase activity and the capacity for bicarbonate oxidation in photosystem II. In: Biochemistry. 2006 ; Vol. 45, No. 7. pp. 2094-2102.
@article{2508944d8ea2491598e9c95b591edd9b,
title = "Quantitative assessment of intrinsic carbonic anhydrase activity and the capacity for bicarbonate oxidation in photosystem II",
abstract = "On the basis of equilibrium isotopic distribution experiments using 18O-labeled water, it is generally accepted that water is the sole substrate for O2 production by photosystem II (PSII). Nevertheless, recent studies indicating a direct interaction between bicarbonate and the donor side of PSII have been used to hypothesize that bicarbonate may have been a physiologically important substrate for O2 production during the evolution of PSII [Dismukes, G. C., Klimov, V. V., Baranov, S. V., Kozlov, Y. N., DasGupta, J., and Tyryshikin, A. (2001) Proc. Natl. Acad. Sci. U.S.A. 98, 2170-2175]. To test out this hypothesis and to determine whether contemporary oxygenic organisms have the capacity to oxidize bicarbonate, we employed special rapid-mixing isotopic experiments using 18O/13C-labeled bicarbonate to quantify the inherent carbonic anhydrase activity in PSII samples and the potential flux of oxygen from bicarbonate into the photosynthetically produced O2. The measurements were made on PSII samples prepared from spinach, Thermosynechococcus elongatus, and Arthrospira maxima. For the latter organism, a strain was used that grows naturally in an alkaline, high (bi)carbonate soda lake in Africa. The results reveal that bicarbonate is not the substrate for O2 production in these contemporary oxygenic photoautotrophs when assayed under single turnover conditions.",
author = "Warwick Hillier and Iain McConnell and Badger, {Murray R.} and Alain Boussac and Klimov, {Vyacheslav V.} and Dismukes, {G Charles} and Tom Wydrzynski",
year = "2006",
month = "2",
day = "21",
doi = "10.1021/bi051892o",
language = "English",
volume = "45",
pages = "2094--2102",
journal = "Biochemistry",
issn = "0006-2960",
publisher = "American Chemical Society",
number = "7",

}

TY - JOUR

T1 - Quantitative assessment of intrinsic carbonic anhydrase activity and the capacity for bicarbonate oxidation in photosystem II

AU - Hillier, Warwick

AU - McConnell, Iain

AU - Badger, Murray R.

AU - Boussac, Alain

AU - Klimov, Vyacheslav V.

AU - Dismukes, G Charles

AU - Wydrzynski, Tom

PY - 2006/2/21

Y1 - 2006/2/21

N2 - On the basis of equilibrium isotopic distribution experiments using 18O-labeled water, it is generally accepted that water is the sole substrate for O2 production by photosystem II (PSII). Nevertheless, recent studies indicating a direct interaction between bicarbonate and the donor side of PSII have been used to hypothesize that bicarbonate may have been a physiologically important substrate for O2 production during the evolution of PSII [Dismukes, G. C., Klimov, V. V., Baranov, S. V., Kozlov, Y. N., DasGupta, J., and Tyryshikin, A. (2001) Proc. Natl. Acad. Sci. U.S.A. 98, 2170-2175]. To test out this hypothesis and to determine whether contemporary oxygenic organisms have the capacity to oxidize bicarbonate, we employed special rapid-mixing isotopic experiments using 18O/13C-labeled bicarbonate to quantify the inherent carbonic anhydrase activity in PSII samples and the potential flux of oxygen from bicarbonate into the photosynthetically produced O2. The measurements were made on PSII samples prepared from spinach, Thermosynechococcus elongatus, and Arthrospira maxima. For the latter organism, a strain was used that grows naturally in an alkaline, high (bi)carbonate soda lake in Africa. The results reveal that bicarbonate is not the substrate for O2 production in these contemporary oxygenic photoautotrophs when assayed under single turnover conditions.

AB - On the basis of equilibrium isotopic distribution experiments using 18O-labeled water, it is generally accepted that water is the sole substrate for O2 production by photosystem II (PSII). Nevertheless, recent studies indicating a direct interaction between bicarbonate and the donor side of PSII have been used to hypothesize that bicarbonate may have been a physiologically important substrate for O2 production during the evolution of PSII [Dismukes, G. C., Klimov, V. V., Baranov, S. V., Kozlov, Y. N., DasGupta, J., and Tyryshikin, A. (2001) Proc. Natl. Acad. Sci. U.S.A. 98, 2170-2175]. To test out this hypothesis and to determine whether contemporary oxygenic organisms have the capacity to oxidize bicarbonate, we employed special rapid-mixing isotopic experiments using 18O/13C-labeled bicarbonate to quantify the inherent carbonic anhydrase activity in PSII samples and the potential flux of oxygen from bicarbonate into the photosynthetically produced O2. The measurements were made on PSII samples prepared from spinach, Thermosynechococcus elongatus, and Arthrospira maxima. For the latter organism, a strain was used that grows naturally in an alkaline, high (bi)carbonate soda lake in Africa. The results reveal that bicarbonate is not the substrate for O2 production in these contemporary oxygenic photoautotrophs when assayed under single turnover conditions.

UR - http://www.scopus.com/inward/record.url?scp=33144472321&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33144472321&partnerID=8YFLogxK

U2 - 10.1021/bi051892o

DO - 10.1021/bi051892o

M3 - Article

VL - 45

SP - 2094

EP - 2102

JO - Biochemistry

JF - Biochemistry

SN - 0006-2960

IS - 7

ER -