We used DNA origami as a platform to coassemble a 20 nm gold nanoparticle (AuNP) and an organic fluorophore (TAMRA) and studied the distance-dependent plasmonic interactions between the particle and the dye using steady state fluorescence and lifetime measurements. Greater fluorescence quenching was found at smaller dye-particle distances, which was accompanied by an enhancement of the decay rate. We also fabricated 20 and 30 nm AuNP homodimers using DNA origami scaffolds and positioned a Cy3 fluorophore between the AuNPs in both assemblies. For each particle size, three different interparticle distances were investigated. Up to 50% enhancement of the Cy3 fluorescence quantum efficiency was observed for the dye between the 30 nm AuNPs. These results are in good agreement with the theoretical simulations.
ASJC Scopus subject areas
- Electronic, Optical and Magnetic Materials
- Physical and Theoretical Chemistry
- Surfaces, Coatings and Films