Radiation damage in protein serial femtosecond crystallography using an x-ray free-electron laser

Lukas Lomb, Thomas R M Barends, Stephan Kassemeyer, Andrew Aquila, Sascha W. Epp, Benjamin Erk, Lutz Foucar, Robert Hartmann, Benedikt Rudek, Daniel Rolles, Artem Rudenko, Robert L. Shoeman, Jakob Andreasson, Sasa Bajt, Miriam Barthelmess, Anton Barty, Michael J. Bogan, Christoph Bostedt, John D. Bozek, Carl Caleman & 45 others Ryan Coffee, Nicola Coppola, Daniel P. Deponte, R. Bruce Doak, Tomas Ekeberg, Holger Fleckenstein, Petra Fromme, Maike Gebhardt, Heinz Graafsma, Lars Gumprecht, Christina Y. Hampton, Andreas Hartmann, Günter Hauser, Helmut Hirsemann, Peter Holl, James M. Holton, Mark S. Hunter, Wolfgang Kabsch, Nils Kimmel, Richard A. Kirian, Mengning Liang, Filipe R N C Maia, Anton Meinhart, Stefano Marchesini, Andrew V. Martin, Karol Nass, Christian Reich, Joachim Schulz, M. Marvin Seibert, Raymond Sierra, Heike Soltau, John C H Spence, Jan Steinbrener, Francesco Stellato, Stephan Stern, Nicusor Timneanu, Xiaoyu Wang, Georg Weidenspointner, Uwe Weierstall, Thomas A. White, Cornelia Wunderer, Henry N. Chapman, Joachim Ullrich, Lothar Strüder, Ilme Schlichting

Research output: Contribution to journalArticle

111 Citations (Scopus)

Abstract

X-ray free-electron lasers deliver intense femtosecond pulses that promise to yield high resolution diffraction data of nanocrystals before the destruction of the sample by radiation damage. Diffraction intensities of lysozyme nanocrystals collected at the Linac Coherent Light Source using 2 keV photons were used for structure determination by molecular replacement and analyzed for radiation damage as a function of pulse length and fluence. Signatures of radiation damage are observed for pulses as short as 70 fs. Parametric scaling used in conventional crystallography does not account for the observed effects.

Original languageEnglish
Article number214111
JournalPhysical Review B - Condensed Matter and Materials Physics
Volume84
Issue number21
DOIs
Publication statusPublished - Dec 20 2011

Fingerprint

x ray lasers
Crystallography
Free electron lasers
Radiation damage
radiation damage
free electron lasers
crystallography
proteins
Proteins
X rays
Nanocrystals
nanocrystals
Diffraction
pulses
X ray lasers
coherent light
lysozyme
Muramidase
Ultrashort pulses
diffraction

ASJC Scopus subject areas

  • Condensed Matter Physics
  • Electronic, Optical and Magnetic Materials

Cite this

Radiation damage in protein serial femtosecond crystallography using an x-ray free-electron laser. / Lomb, Lukas; Barends, Thomas R M; Kassemeyer, Stephan; Aquila, Andrew; Epp, Sascha W.; Erk, Benjamin; Foucar, Lutz; Hartmann, Robert; Rudek, Benedikt; Rolles, Daniel; Rudenko, Artem; Shoeman, Robert L.; Andreasson, Jakob; Bajt, Sasa; Barthelmess, Miriam; Barty, Anton; Bogan, Michael J.; Bostedt, Christoph; Bozek, John D.; Caleman, Carl; Coffee, Ryan; Coppola, Nicola; Deponte, Daniel P.; Doak, R. Bruce; Ekeberg, Tomas; Fleckenstein, Holger; Fromme, Petra; Gebhardt, Maike; Graafsma, Heinz; Gumprecht, Lars; Hampton, Christina Y.; Hartmann, Andreas; Hauser, Günter; Hirsemann, Helmut; Holl, Peter; Holton, James M.; Hunter, Mark S.; Kabsch, Wolfgang; Kimmel, Nils; Kirian, Richard A.; Liang, Mengning; Maia, Filipe R N C; Meinhart, Anton; Marchesini, Stefano; Martin, Andrew V.; Nass, Karol; Reich, Christian; Schulz, Joachim; Seibert, M. Marvin; Sierra, Raymond; Soltau, Heike; Spence, John C H; Steinbrener, Jan; Stellato, Francesco; Stern, Stephan; Timneanu, Nicusor; Wang, Xiaoyu; Weidenspointner, Georg; Weierstall, Uwe; White, Thomas A.; Wunderer, Cornelia; Chapman, Henry N.; Ullrich, Joachim; Strüder, Lothar; Schlichting, Ilme.

In: Physical Review B - Condensed Matter and Materials Physics, Vol. 84, No. 21, 214111, 20.12.2011.

Research output: Contribution to journalArticle

Lomb, L, Barends, TRM, Kassemeyer, S, Aquila, A, Epp, SW, Erk, B, Foucar, L, Hartmann, R, Rudek, B, Rolles, D, Rudenko, A, Shoeman, RL, Andreasson, J, Bajt, S, Barthelmess, M, Barty, A, Bogan, MJ, Bostedt, C, Bozek, JD, Caleman, C, Coffee, R, Coppola, N, Deponte, DP, Doak, RB, Ekeberg, T, Fleckenstein, H, Fromme, P, Gebhardt, M, Graafsma, H, Gumprecht, L, Hampton, CY, Hartmann, A, Hauser, G, Hirsemann, H, Holl, P, Holton, JM, Hunter, MS, Kabsch, W, Kimmel, N, Kirian, RA, Liang, M, Maia, FRNC, Meinhart, A, Marchesini, S, Martin, AV, Nass, K, Reich, C, Schulz, J, Seibert, MM, Sierra, R, Soltau, H, Spence, JCH, Steinbrener, J, Stellato, F, Stern, S, Timneanu, N, Wang, X, Weidenspointner, G, Weierstall, U, White, TA, Wunderer, C, Chapman, HN, Ullrich, J, Strüder, L & Schlichting, I 2011, 'Radiation damage in protein serial femtosecond crystallography using an x-ray free-electron laser', Physical Review B - Condensed Matter and Materials Physics, vol. 84, no. 21, 214111. https://doi.org/10.1103/PhysRevB.84.214111
Lomb, Lukas ; Barends, Thomas R M ; Kassemeyer, Stephan ; Aquila, Andrew ; Epp, Sascha W. ; Erk, Benjamin ; Foucar, Lutz ; Hartmann, Robert ; Rudek, Benedikt ; Rolles, Daniel ; Rudenko, Artem ; Shoeman, Robert L. ; Andreasson, Jakob ; Bajt, Sasa ; Barthelmess, Miriam ; Barty, Anton ; Bogan, Michael J. ; Bostedt, Christoph ; Bozek, John D. ; Caleman, Carl ; Coffee, Ryan ; Coppola, Nicola ; Deponte, Daniel P. ; Doak, R. Bruce ; Ekeberg, Tomas ; Fleckenstein, Holger ; Fromme, Petra ; Gebhardt, Maike ; Graafsma, Heinz ; Gumprecht, Lars ; Hampton, Christina Y. ; Hartmann, Andreas ; Hauser, Günter ; Hirsemann, Helmut ; Holl, Peter ; Holton, James M. ; Hunter, Mark S. ; Kabsch, Wolfgang ; Kimmel, Nils ; Kirian, Richard A. ; Liang, Mengning ; Maia, Filipe R N C ; Meinhart, Anton ; Marchesini, Stefano ; Martin, Andrew V. ; Nass, Karol ; Reich, Christian ; Schulz, Joachim ; Seibert, M. Marvin ; Sierra, Raymond ; Soltau, Heike ; Spence, John C H ; Steinbrener, Jan ; Stellato, Francesco ; Stern, Stephan ; Timneanu, Nicusor ; Wang, Xiaoyu ; Weidenspointner, Georg ; Weierstall, Uwe ; White, Thomas A. ; Wunderer, Cornelia ; Chapman, Henry N. ; Ullrich, Joachim ; Strüder, Lothar ; Schlichting, Ilme. / Radiation damage in protein serial femtosecond crystallography using an x-ray free-electron laser. In: Physical Review B - Condensed Matter and Materials Physics. 2011 ; Vol. 84, No. 21.
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abstract = "X-ray free-electron lasers deliver intense femtosecond pulses that promise to yield high resolution diffraction data of nanocrystals before the destruction of the sample by radiation damage. Diffraction intensities of lysozyme nanocrystals collected at the Linac Coherent Light Source using 2 keV photons were used for structure determination by molecular replacement and analyzed for radiation damage as a function of pulse length and fluence. Signatures of radiation damage are observed for pulses as short as 70 fs. Parametric scaling used in conventional crystallography does not account for the observed effects.",
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AU - Foucar, Lutz

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AU - Hauser, Günter

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AU - Hunter, Mark S.

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AU - Kirian, Richard A.

AU - Liang, Mengning

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