Rational design of a hexameric protein assembly stabilized by metal chelation

Rafael Alcala-Torano, Mathieu Walther, Dayn J. Sommer, Chad K. Park, Giovanna Ghirlanda

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Protein-based self-assembled nanostructures hold tremendous promise as smart materials. One strategy to control the assembly of individual protein modules takes advantage of the directionality and high affinity bonding afforded by metal chelation. Here, we describe the use of 2,2′-bipyridine units (Bpy) as side chains to template the assembly of large structures (MW approx. 35 000 Da) in a metal-dependent manner. The structures are trimers of independently folded 3-helix bundles, and are held together by 2 Me(Bpy)3 complexes. The assemblies are stable to thermal denaturation, and are more than 90% helical at 90°C. Circular dichroism spectroscopy shows that one of the 2 possible (Bpy)3 enantiomers is favored over the other. Because of the sequence pliability of the starting peptides, these constructs could find use to organize functional groups at controlled positions within a supramolecular assembly.

Original languageEnglish
Article numbere23233
JournalBiopolymers
Volume109
Issue number10
DOIs
Publication statusPublished - Aug 1 2018

Fingerprint

Chelation
Metals
Proteins
Nanostructures
Circular Dichroism
Pliability
Spectrum Analysis
Hot Temperature
Circular dichroism spectroscopy
Peptides
Denaturation
Intelligent materials
Enantiomers
Functional groups

Keywords

  • metal binding
  • protein assembly
  • protein design

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Biomaterials
  • Organic Chemistry

Cite this

Rational design of a hexameric protein assembly stabilized by metal chelation. / Alcala-Torano, Rafael; Walther, Mathieu; Sommer, Dayn J.; Park, Chad K.; Ghirlanda, Giovanna.

In: Biopolymers, Vol. 109, No. 10, e23233, 01.08.2018.

Research output: Contribution to journalArticle

Alcala-Torano, Rafael ; Walther, Mathieu ; Sommer, Dayn J. ; Park, Chad K. ; Ghirlanda, Giovanna. / Rational design of a hexameric protein assembly stabilized by metal chelation. In: Biopolymers. 2018 ; Vol. 109, No. 10.
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