Reactions of Nitric Oxide with Cytochrome C Oxidase

Gary W. Brudvig, Tom H. Stevens, Sunney I. Chan

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209 Citations (Scopus)

Abstract

The reactions of nitric oxide (NO) with both oxidized and reduced cytochrome c oxidase are reported. NMR and mass spectroscopy were utilized to determine the products of the reactions; EPR and optical spectroscopy were employed to determine the states of the enzyme produced in each of these reactions. It was found that the enzyme catalyzes the consecutive oxidation and reduction of NO. A different cycle was observed when NO was added to the reduced enzyme, to the oxidized enzyme, or to the oxidized enzyme in the presence of azide.It was possible to observe the state of the enzyme at several points in each of these three cycles by varying the concentration of NO. The reactions of NO all involved a one- or two-electron redox step and could be accounted for by the involvement of only cytochrome a3and Cua3.On the basis of these results, a mechanism for the reduction of dioxygen by the enzyme is proposed in which cytochrome a3functions to anchor dioxygen and intermediates while remaining in the ferrous state, whereas Cua3functions to accept electrons from cytochrome a/Cuaand transfer them to dioxygen.

Original languageEnglish
Pages (from-to)5275-5285
Number of pages11
JournalBiochemistry
Volume19
Issue number23
DOIs
Publication statusPublished - 1980

ASJC Scopus subject areas

  • Biochemistry

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