Recognition imaging with a DNA aptamer

Liyun Lin, Hongda Wang, Yan Liu, Hao Yan, Stuart Lindsay

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

We have used a DNA-aptamer tethered to an atomic force microscope probe to carry out recognition imaging of IgE molecules attached to a mica substrate. The recognition was efficient (∼90%) and specific, being blocked by injection of IgE molecules in solution, and not being interfered with by high concentrations of a second protein. The signal/noise ratio of the recognition signal was better than that obtained with antibodies, despite the fact that the average force required to break the aptamer-protein bonds was somewhat smaller.

Original languageEnglish
Pages (from-to)4236-4238
Number of pages3
JournalBiophysical journal
Volume90
Issue number11
DOIs
Publication statusPublished - Jun 2006

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ASJC Scopus subject areas

  • Biophysics

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