Recognition imaging with a DNA aptamer

Liyun Lin, Hongda Wang, Yan Liu, Hao Yan, Stuart Lindsay

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

We have used a DNA-aptamer tethered to an atomic force microscope probe to carry out recognition imaging of IgE molecules attached to a mica substrate. The recognition was efficient (∼90%) and specific, being blocked by injection of IgE molecules in solution, and not being interfered with by high concentrations of a second protein. The signal/noise ratio of the recognition signal was better than that obtained with antibodies, despite the fact that the average force required to break the aptamer-protein bonds was somewhat smaller.

Original languageEnglish
Pages (from-to)4236-4238
Number of pages3
JournalBiophysical Journal
Volume90
Issue number11
DOIs
Publication statusPublished - Jun 2006

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Nucleotide Aptamers
Immunoglobulin E
Proteins
Injections
Antibodies
mica

ASJC Scopus subject areas

  • Biophysics

Cite this

Recognition imaging with a DNA aptamer. / Lin, Liyun; Wang, Hongda; Liu, Yan; Yan, Hao; Lindsay, Stuart.

In: Biophysical Journal, Vol. 90, No. 11, 06.2006, p. 4236-4238.

Research output: Contribution to journalArticle

Lin, Liyun ; Wang, Hongda ; Liu, Yan ; Yan, Hao ; Lindsay, Stuart. / Recognition imaging with a DNA aptamer. In: Biophysical Journal. 2006 ; Vol. 90, No. 11. pp. 4236-4238.
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