Reduced Occupancy of the Oxygen-Evolving Complex of Photosystem II Detected in Cryo-Electron Microscopy Maps

Jimin Wang, Krystle Reiss, Gary W Brudvig, Victor S. Batista

Research output: Contribution to journalArticle

Abstract

Computational simulations of electrostatic potentials (ESPs), based on atomistic models and independent atomic scattering factors, have remained challenging when applied to the oxygen-evolving complex (OEC) of photosystem II (PSII). Here, we overcome that challenge by using an ESP function obtained with density functional theory and atomic coordinates for the OEC of PSII obtained by optimization of the dark-adapted S1 state. We find that the ESP is much higher for the OEC than for the nearby reference side chain of amino acid residue D1-H190. In contrast, experimental ESP maps recently published for two PSII-light-harvesting complex II super-complexes show that the ESP of the OEC is approximately half the value of the D1-H190 side chain. The apparent disparity is attributed to a reduced 31-38% occupancy of the OEC, likely associated with its reduction by electron scattering.

Original languageEnglish
Pages (from-to)5925-5929
Number of pages5
JournalBiochemistry
Volume57
Issue number41
DOIs
Publication statusPublished - Oct 16 2018

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Cryoelectron Microscopy
Photosystem II Protein Complex
Static Electricity
Electron microscopy
Electrostatics
Oxygen
Electron scattering
Lattice vibrations
Density functional theory
Electrons
Light
Amino Acids

ASJC Scopus subject areas

  • Biochemistry

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Reduced Occupancy of the Oxygen-Evolving Complex of Photosystem II Detected in Cryo-Electron Microscopy Maps. / Wang, Jimin; Reiss, Krystle; Brudvig, Gary W; Batista, Victor S.

In: Biochemistry, Vol. 57, No. 41, 16.10.2018, p. 5925-5929.

Research output: Contribution to journalArticle

Wang, Jimin ; Reiss, Krystle ; Brudvig, Gary W ; Batista, Victor S. / Reduced Occupancy of the Oxygen-Evolving Complex of Photosystem II Detected in Cryo-Electron Microscopy Maps. In: Biochemistry. 2018 ; Vol. 57, No. 41. pp. 5925-5929.
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