Reevaluation of the stoichiometry of cytochrome b559 in photosystem II and thylakoid membranes

Carolyn A. Buser, Gary W Brudvig, Gary W. Brudvig

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

The stoichiometry of cytochrome b559 (one or two copies) per reaction center of photosystem II (PSII) has been the subject of considerable debate. The molar ratio of cytochrome b559 has a number of significant implications on our understanding of the functional role of cytochrome b559, the mechanism of electron donation in PSII, and the stoichiometry of the other redox-active, reaction center components. We have reinvestigated the stoichiometry of cytochrome b559 in PSII-enriched and thylakoid membranes, using differential absorbance and electron paramagnetic resonance spectroscopies. The data from both quantitation procedures strongly indicate only one copy of cytochrome b559 per reaction center in PSII-enriched membranes and also suggest one copy of cytochrome b559 per reaction center in thylakoid membranes.

Original languageEnglish
Pages (from-to)11441-11448
Number of pages8
JournalBiochemistry
Volume31
Issue number46
Publication statusPublished - 1992

Fingerprint

Thylakoids
Photosystem II Protein Complex
Stoichiometry
Membranes
Electron Spin Resonance Spectroscopy
Oxidation-Reduction
Paramagnetic resonance
cytochrome b559
Spectrum Analysis
Spectroscopy
Electrons

ASJC Scopus subject areas

  • Biochemistry

Cite this

Reevaluation of the stoichiometry of cytochrome b559 in photosystem II and thylakoid membranes. / Buser, Carolyn A.; Brudvig, Gary W; Brudvig, Gary W.

In: Biochemistry, Vol. 31, No. 46, 1992, p. 11441-11448.

Research output: Contribution to journalArticle

Buser, Carolyn A. ; Brudvig, Gary W ; Brudvig, Gary W. / Reevaluation of the stoichiometry of cytochrome b559 in photosystem II and thylakoid membranes. In: Biochemistry. 1992 ; Vol. 31, No. 46. pp. 11441-11448.
@article{f65c726e988042b5977f870bcfc7a370,
title = "Reevaluation of the stoichiometry of cytochrome b559 in photosystem II and thylakoid membranes",
abstract = "The stoichiometry of cytochrome b559 (one or two copies) per reaction center of photosystem II (PSII) has been the subject of considerable debate. The molar ratio of cytochrome b559 has a number of significant implications on our understanding of the functional role of cytochrome b559, the mechanism of electron donation in PSII, and the stoichiometry of the other redox-active, reaction center components. We have reinvestigated the stoichiometry of cytochrome b559 in PSII-enriched and thylakoid membranes, using differential absorbance and electron paramagnetic resonance spectroscopies. The data from both quantitation procedures strongly indicate only one copy of cytochrome b559 per reaction center in PSII-enriched membranes and also suggest one copy of cytochrome b559 per reaction center in thylakoid membranes.",
author = "Buser, {Carolyn A.} and Brudvig, {Gary W} and Brudvig, {Gary W.}",
year = "1992",
language = "English",
volume = "31",
pages = "11441--11448",
journal = "Biochemistry",
issn = "0006-2960",
publisher = "American Chemical Society",
number = "46",

}

TY - JOUR

T1 - Reevaluation of the stoichiometry of cytochrome b559 in photosystem II and thylakoid membranes

AU - Buser, Carolyn A.

AU - Brudvig, Gary W

AU - Brudvig, Gary W.

PY - 1992

Y1 - 1992

N2 - The stoichiometry of cytochrome b559 (one or two copies) per reaction center of photosystem II (PSII) has been the subject of considerable debate. The molar ratio of cytochrome b559 has a number of significant implications on our understanding of the functional role of cytochrome b559, the mechanism of electron donation in PSII, and the stoichiometry of the other redox-active, reaction center components. We have reinvestigated the stoichiometry of cytochrome b559 in PSII-enriched and thylakoid membranes, using differential absorbance and electron paramagnetic resonance spectroscopies. The data from both quantitation procedures strongly indicate only one copy of cytochrome b559 per reaction center in PSII-enriched membranes and also suggest one copy of cytochrome b559 per reaction center in thylakoid membranes.

AB - The stoichiometry of cytochrome b559 (one or two copies) per reaction center of photosystem II (PSII) has been the subject of considerable debate. The molar ratio of cytochrome b559 has a number of significant implications on our understanding of the functional role of cytochrome b559, the mechanism of electron donation in PSII, and the stoichiometry of the other redox-active, reaction center components. We have reinvestigated the stoichiometry of cytochrome b559 in PSII-enriched and thylakoid membranes, using differential absorbance and electron paramagnetic resonance spectroscopies. The data from both quantitation procedures strongly indicate only one copy of cytochrome b559 per reaction center in PSII-enriched membranes and also suggest one copy of cytochrome b559 per reaction center in thylakoid membranes.

UR - http://www.scopus.com/inward/record.url?scp=0026490910&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026490910&partnerID=8YFLogxK

M3 - Article

VL - 31

SP - 11441

EP - 11448

JO - Biochemistry

JF - Biochemistry

SN - 0006-2960

IS - 46

ER -