Regulatory branch points affecting protein and lipid biosynthesis in the diatom Phaeodactylum tricornutum

L. Tiago Guerra, Orly Levitan, Miguel J. Frada, Jennifer S. Sun, Paul G. Falkowski, G Charles Dismukes

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

It is widely established that nutritional nitrogen deprivation increases lipid accumulation but severely decreases growth rate in microalgae. To understand the regulatory branch points that determine the partitioning of carbon among its potential sinks, we analyzed metabolite and transcript levels of central carbon metabolic pathways and determined the average fluxes and quantum requirements for the synthesis of protein, carbohydrates and fatty acid in the diatom Phaeodactylum tricornutum. Under nitrate-starved conditions, the carbon fluxes into all major sinks decrease sharply; the largest decrease was into proteins and smallest was into lipids. This reduction of carbon flux into lipids together with a significantly lower growth rate is responsible for lower overall FA productivities implying that nitrogen starvation is not a bioenergetically feasible strategy for increasing biodiesel production. The reduction in these fluxes was accompanied by an 18-fold increase in α-ketoglutarate (AKG), 3-fold increase in NADPH/NADP+, and sharp decreases in glutamate (GLU) and glutamine (GLN) levels. Additionally, the mRNA level of acetyl-CoA carboxylase and two type II diacylglycerol-acyltransferases were increased. Partial suppression of nitrate reductase by tungstate resulted in similar trends at lower levels as for nitrate starvation. These results reveal that the GS/GOGAT pathway is the main regulation site for nitrate dependent control of carbon partitioning between protein and lipid biosynthesis, while the AKG/GL(N/U) metabolite ratio is a transcriptional signal, possibly related to redox poise of intermediates in the photosynthetic electron transport system.

Original languageEnglish
Pages (from-to)306-315
Number of pages10
JournalBiomass and Bioenergy
Volume59
DOIs
Publication statusPublished - Dec 2013

Fingerprint

Phaeodactylum tricornutum
Bacillariophyceae
Biosynthesis
Lipids
diatom
lipid
biosynthesis
nitrate
Nitrates
Proteins
Carbon
protein
carbon
Fluxes
carbon flux
lipids
starvation
nitrates
metabolite
Metabolites

Keywords

  • Biofuels
  • Carbon partition
  • GS/GOGAT
  • Lipid
  • Microalgae
  • Nitrogen metabolism

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Forestry
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal

Cite this

Regulatory branch points affecting protein and lipid biosynthesis in the diatom Phaeodactylum tricornutum. / Guerra, L. Tiago; Levitan, Orly; Frada, Miguel J.; Sun, Jennifer S.; Falkowski, Paul G.; Dismukes, G Charles.

In: Biomass and Bioenergy, Vol. 59, 12.2013, p. 306-315.

Research output: Contribution to journalArticle

Guerra, L. Tiago ; Levitan, Orly ; Frada, Miguel J. ; Sun, Jennifer S. ; Falkowski, Paul G. ; Dismukes, G Charles. / Regulatory branch points affecting protein and lipid biosynthesis in the diatom Phaeodactylum tricornutum. In: Biomass and Bioenergy. 2013 ; Vol. 59. pp. 306-315.
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