Resolving Single-Nanoconstruct Dynamics during Targeting and Nontargeting Live-Cell Membrane Interactions

Debanjan Bhowmik, Kayla S.B. Culver, Tingting Liu, Teri W. Odom

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

This paper describes how differences in the dynamics of targeting and nontargeting constructs can provide information on nanoparticle (NP)-cell interactions. We probed translational and rotational dynamics of functionalized Au nanostar (AuNS) nanoconstructs interacting with cells in serum-containing medium. We found that AuNS with targeting ligands had a larger dynamical footprint and faster rotational speed on cell membranes expressing human epidermal growth factor receptor 2 (HER-2) receptors compared to that of AuNS with nontargeting ligands. Targeting and nontargeting nanoconstructs displayed distinct membrane dynamics despite their similar protein adsorption profiles, which suggests that targeted interactions are preserved even in the presence of a protein corona. The high sensitivity of single-NP dynamics can be used to compare different nanoconstruct properties (such as NP size, shape, and surface chemistry) to improve their design as delivery vehicles.

Original languageEnglish
Pages (from-to)13637-13644
Number of pages8
JournalACS nano
Volume13
Issue number12
DOIs
Publication statusPublished - Dec 24 2019

Keywords

  • differential interference contrast microscopy
  • membrane-receptor interactions
  • protein corona
  • single-particle dynamics
  • targeting and nontargeting nanoconstructs

ASJC Scopus subject areas

  • Materials Science(all)
  • Engineering(all)
  • Physics and Astronomy(all)

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