Resonance localized surface plasmon spectroscopy: Sensing substrate and inhibitor binding to cytochrome P450

Jing Zhao, Aditi Das, George C Schatz, Stephen G. Sligar, Richard P. Van Duyne

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

A sensing method based on resonance localized surface plasmon spectroscopy was developed for low molecular weight substrate and inhibitor molecules binding to heme proteins. Cytochrome P450 proteins have Soret and Q absorption bands in the visible wavelength region. The coupling between the molecular resonance of P450 and the localized surface plasmon resonance (LSPR) of functionalized silver nanoparticles leads to a highly wavelength-dependent LSPR response. Binding of substrate (e.g., camphor) or inhibitor (e.g., imidazole) molecules to a cytochrome P450 causes the absorption band of cytochrome P450 shift to shorter or longer wavelengths, respectively. By monitoring the localized surface plasmon resonance (LSPR) of the nanosensors, the binding of camphor/imidazole to a nanoparticle whose surface is modified with cytochrome P450 protein leads to a wavelength-dependent blue/red shift in the LSPR. The magnitude of the LSPR shift induced by camphor or imidazole is consistent with the Soret band wavelength shift observed in P450 in solution.

Original languageEnglish
Pages (from-to)13084-13088
Number of pages5
JournalJournal of Physical Chemistry C
Volume112
Issue number34
DOIs
Publication statusPublished - Aug 28 2008

Fingerprint

cytochromes
Surface plasmon resonance
surface plasmon resonance
inhibitors
Cytochrome P-450 Enzyme System
Spectroscopy
Camphor
camphor
Substrates
Wavelength
imidazoles
spectroscopy
wavelengths
proteins
Absorption spectra
shift
Nanosensors
Nanoparticles
Hemeproteins
Proteins

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Electronic, Optical and Magnetic Materials
  • Surfaces, Coatings and Films
  • Energy(all)

Cite this

Resonance localized surface plasmon spectroscopy : Sensing substrate and inhibitor binding to cytochrome P450. / Zhao, Jing; Das, Aditi; Schatz, George C; Sligar, Stephen G.; Van Duyne, Richard P.

In: Journal of Physical Chemistry C, Vol. 112, No. 34, 28.08.2008, p. 13084-13088.

Research output: Contribution to journalArticle

Zhao, Jing ; Das, Aditi ; Schatz, George C ; Sligar, Stephen G. ; Van Duyne, Richard P. / Resonance localized surface plasmon spectroscopy : Sensing substrate and inhibitor binding to cytochrome P450. In: Journal of Physical Chemistry C. 2008 ; Vol. 112, No. 34. pp. 13084-13088.
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