Resonance Raman spectroscopy of carotenoids in Photosystem II core complexes

Cara A. Tracewell, Agnes Cua, David F. Bocian, Gary W Brudvig

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Resonance Raman (RR) spectroscopy has been used to examine the configuration of the carotenoids bound to Synechocystis PCC 6803 Photosystem II (PS II) core complexes. The excitation wavelengths used (514.5, 488.0, 476.5 and 457.9 nm) span the absorption bands of all of the ∼12-17 neutral carotenoids in the PS II core complex. The RR spectra of the two carotenoids associated with the D1-D2 polypeptides (Car507 and Car489) of the reaction center are extracted via light versus dark difference experiments measured at 20 K. The RR results are consistent with all-trans configurations for both Car507 and Car489 and indicate that majority of the other carotenoids in the PS II core complex must also be in the all-trans configuration. The configuration of β-carotene is relevant to its proposed function as a molecular wire in the secondary electron-transfer reactions of PS II.

Original languageEnglish
Pages (from-to)45-52
Number of pages8
JournalPhotosynthesis Research
Volume83
Issue number1
DOIs
Publication statusPublished - Jan 2005

Fingerprint

Raman spectroscopy
Photosystem II Protein Complex
Raman Spectrum Analysis
Carotenoids
photosystem II
carotenoids
nanowires
Synechocystis
carotenes
electron transfer
wavelengths
polypeptides
Raman scattering
Absorption spectra
Wire
Electrons
Light
Wavelength
Peptides
Experiments

Keywords

  • β-carotene
  • Photosystem II
  • Resonance Raman
  • Secondary electron transfer pathway

ASJC Scopus subject areas

  • Plant Science

Cite this

Resonance Raman spectroscopy of carotenoids in Photosystem II core complexes. / Tracewell, Cara A.; Cua, Agnes; Bocian, David F.; Brudvig, Gary W.

In: Photosynthesis Research, Vol. 83, No. 1, 01.2005, p. 45-52.

Research output: Contribution to journalArticle

Tracewell, Cara A. ; Cua, Agnes ; Bocian, David F. ; Brudvig, Gary W. / Resonance Raman spectroscopy of carotenoids in Photosystem II core complexes. In: Photosynthesis Research. 2005 ; Vol. 83, No. 1. pp. 45-52.
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