Robust DNA-functionalized core/shell quantum dots with fluorescent emission spanning from UV-vis to near-IR and compatible with DNA-directed self-assembly

Zhengtao Deng, Anirban Samanta, Jeanette Nangreave, Hao Yan, Yan Liu

Research output: Contribution to journalArticle

77 Citations (Scopus)

Abstract

The assembly and isolation of DNA oligonucleotide-functionalized gold nanoparticles (AuNPs) has become a well-developed technology that is based on the strong bonding interactions between gold and thiolated DNA. However, achieving DNA-functionalized semiconductor quantum dots (QDs) that are robust enough to withstand precipitation at high temperature and ionic strength through simple attachment of modified DNA to the QD surface remains a challenge. We report the synthesis of stable core/shell (1-20 monolayers) QD-DNA conjugates in which the end of the phosphorothiolated oligonucleotide (5-10 nucleotides) is "embedded" within the shell of the QD. These reliable QD-DNA conjugates exhibit excellent chemical and photonic stability, colloidal stability over a wide pH range (4-12) and at high salt concentrations (>100 mM Na+ or Mg2+), bright fluorescence emission with quantum yields of up to 70%, and broad spectral tunability with emission ranging from the UV to the NIR (360-800 nm).

Original languageEnglish
Pages (from-to)17424-17427
Number of pages4
JournalJournal of the American Chemical Society
Volume134
Issue number42
DOIs
Publication statusPublished - Oct 24 2012

Fingerprint

Quantum Dots
Self assembly
Semiconductor quantum dots
DNA
Oligonucleotides
Gold
Optics and Photonics
Semiconductors
Quantum yield
Nucleotides
Ionic strength
Nanoparticles
Osmolar Concentration
Photonics
Monolayers
Salts
Fluorescence
Technology
Temperature

ASJC Scopus subject areas

  • Chemistry(all)
  • Catalysis
  • Biochemistry
  • Colloid and Surface Chemistry

Cite this

Robust DNA-functionalized core/shell quantum dots with fluorescent emission spanning from UV-vis to near-IR and compatible with DNA-directed self-assembly. / Deng, Zhengtao; Samanta, Anirban; Nangreave, Jeanette; Yan, Hao; Liu, Yan.

In: Journal of the American Chemical Society, Vol. 134, No. 42, 24.10.2012, p. 17424-17427.

Research output: Contribution to journalArticle

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