Rolling circle enzymatic replication of a complex multi-crossover DNA nanostructure

Chenxiang Lin, Xing Wang, Yan Liu, Nadrian C. Seeman, Hao Yan

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Nature has evolved replicable biological molecules, such as DNA, as genetic information carriers. The replication process is tightly controlled by complicated cellular machinery. It is interesting to ask if artificial DNA nano-objects with a complex secondary structure can be replicated in the same way as simple DNA double helices. Here we demonstrate that paranemic crossover DNA, a structurally complicated multi-crossover DNA molecule, can be replicated successfully using Rolling Circle Amplification (RCA). The amplification efficiency is moderate with high fidelity, confirmed by native PAGE, thermal transition study, and Ferguson analysis. The structural details of the DNA structure after the full replication circle are verified by hydroxyl radical autofootprinting. We conclude that RCA can serve as a reliable method to replicate complex DNA structures. We also discuss the possibility of using viruses and bacteria to clone artificial DNA nano-objects. The findings that single stranded paranemic crossover DNA molecules can be replicated by DNA polymerase will not only be useful in nanotechnology but also may have implications for the possible existence of such complicated DNA structures in nature.

Original languageEnglish
Pages (from-to)14475-14481
Number of pages7
JournalJournal of the American Chemical Society
Volume129
Issue number46
DOIs
Publication statusPublished - Nov 21 2007

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Nanostructures
DNA
Amplification
Molecules
Native Polyacrylamide Gel Electrophoresis
Nanotechnology
DNA-Directed DNA Polymerase
Heterozygote
Viruses
Hydroxyl Radical
Machinery
Bacteria
Clone Cells
Hot Temperature

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Rolling circle enzymatic replication of a complex multi-crossover DNA nanostructure. / Lin, Chenxiang; Wang, Xing; Liu, Yan; Seeman, Nadrian C.; Yan, Hao.

In: Journal of the American Chemical Society, Vol. 129, No. 46, 21.11.2007, p. 14475-14481.

Research output: Contribution to journalArticle

Lin, Chenxiang ; Wang, Xing ; Liu, Yan ; Seeman, Nadrian C. ; Yan, Hao. / Rolling circle enzymatic replication of a complex multi-crossover DNA nanostructure. In: Journal of the American Chemical Society. 2007 ; Vol. 129, No. 46. pp. 14475-14481.
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