Sample consumption reduction for serial crystallography using water-in-oil droplets

Austin Echelmeier, Jorvani Cruz Villarreal, Daihyn Kim, Sahir Gandhi, Ana Egatz-Gomez, Darren Thifault, Jesse D. Coe, Gerrit Brehm, Caleb Madsen, Sebastian Quintana, Saša Bajt, Marc Messerschmidt, Jose Domingo Meza-Aguilar, Dominik Oberthür, Max O. Wiedorn, Holger Fleckenstein, Sabine Botha, Derek Mendez, Juraj Knoska, Jose Martin GarciaHao Hu, Stella Lisova, Aschkan Allah Gholi, Yaroslav Gevorkov, Kartik Ayyer, Steve J. Aplin, Helen M. Ginn, Heinz Graafsma, Andrew J. Morgan, Dominc Greiffenberg, Alexander Klujev, Torsten Laurus, Jennifer Poehlsen, Ulrich Trunk, Filipe R.N.C. Maia, Davide Mezza, Raimund Fromme, Britta Weinhausen, Grant Mills, Patrik Vagovic, Yoonhee Kim, Joachim Schulz, Katerina Dörner, Jolanta Sztuk-Dambietz, Manuela Kuhn, Thomas D. Grant, Thomas A. White, Valerio Mariani, Anton Barty, Adrian P. Mancuso, Uwe Weierstall, John C.H. Spence, Henry N. Chapman, Nadia A. Zatsepin, Petra Fromme, Richard A. Kirian, Alexandra Ros

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Serial femtosecond crystallography with an X-ray free electron laser (XFEL) wastes up to 99% of the continuously injected sample due to the XFEL pulse structure. To reduce sample waste, we utilize a 3D printed microfluidic droplet generator coupled to an injection nozzle to deliver a segmented flow containing droplets of aqueous crystal suspension within an oil carrier. The structure of 3-deoxy-D-manno-octulosonate 8-phosphate synthase (KDO8PS) was determined and sample waste reduced significantly.

Original languageEnglish
Title of host publication23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
PublisherChemical and Biological Microsystems Society
Pages1476-1477
Number of pages2
ISBN (Electronic)9781733419000
Publication statusPublished - 2019
Event23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019 - Basel, Switzerland
Duration: Oct 27 2019Oct 31 2019

Publication series

Name23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019

Conference

Conference23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
CountrySwitzerland
CityBasel
Period10/27/1910/31/19

Keywords

  • 3D Printing
  • Crystallography
  • Droplets
  • Microfluidics
  • SFX
  • XFEL

ASJC Scopus subject areas

  • Bioengineering
  • Chemical Engineering (miscellaneous)

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