Scanningless depth-resolved microscopy

Dan Oron, Eran Tal, Yaron Silberberg

Research output: Contribution to journalArticle

333 Citations (Scopus)

Abstract

The ability to perform optical sectioning is one of the great advantages of laser-scanning microscopy. This introduces, however, a number of difficulties due to the scanning process, such as lower frame rates due to the serial acquisition process. Here we show that by introducing spatiotemporal pulse shaping techniques to multiphoton microscopy it is possible to obtain full-frame depth resolved imaging completely without scanning. Our method relies on temporal focusing of the illumination pulse. The pulsed excitation field is compressed as it propagates through the sample, reaching its shortest duration at the focal plane, before stretching again beyond it. This method is applied to obtain depth-resolved two-photon excitation fluorescence (TPEF) images of drosophila egg-chambers with nearly 105 effective pixels using a standard Ti:Sapphire laser oscillator.

Original languageEnglish
Pages (from-to)1468-1476
Number of pages9
JournalOptics express
Volume13
Issue number5
DOIs
Publication statusPublished - Mar 2005

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

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