Abstract
The ability to perform optical sectioning is one of the great advantages of laser-scanning microscopy. This introduces, however, a number of difficulties due to the scanning process, such as lower frame rates due to the serial acquisition process. Here we show that by introducing spatiotemporal pulse shaping techniques to multiphoton microscopy it is possible to obtain full-frame depth resolved imaging completely without scanning. Our method relies on temporal focusing of the illumination pulse. The pulsed excitation field is compressed as it propagates through the sample, reaching its shortest duration at the focal plane, before stretching again beyond it. This method is applied to obtain depth-resolved two-photon excitation fluorescence (TPEF) images of drosophila egg-chambers with nearly 105 effective pixels using a standard Ti:Sapphire laser oscillator.
| Original language | English |
|---|---|
| Pages (from-to) | 1468-1476 |
| Number of pages | 9 |
| Journal | Optics Express |
| Volume | 13 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 2005 |
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ASJC Scopus subject areas
- Atomic and Molecular Physics, and Optics
Cite this
Scanningless depth-resolved microscopy. / Oron, Dan; Tal, Eran; Silberberg, Yaron.
In: Optics Express, Vol. 13, No. 5, 2005, p. 1468-1476.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Scanningless depth-resolved microscopy
AU - Oron, Dan
AU - Tal, Eran
AU - Silberberg, Yaron
PY - 2005
Y1 - 2005
N2 - The ability to perform optical sectioning is one of the great advantages of laser-scanning microscopy. This introduces, however, a number of difficulties due to the scanning process, such as lower frame rates due to the serial acquisition process. Here we show that by introducing spatiotemporal pulse shaping techniques to multiphoton microscopy it is possible to obtain full-frame depth resolved imaging completely without scanning. Our method relies on temporal focusing of the illumination pulse. The pulsed excitation field is compressed as it propagates through the sample, reaching its shortest duration at the focal plane, before stretching again beyond it. This method is applied to obtain depth-resolved two-photon excitation fluorescence (TPEF) images of drosophila egg-chambers with nearly 105 effective pixels using a standard Ti:Sapphire laser oscillator.
AB - The ability to perform optical sectioning is one of the great advantages of laser-scanning microscopy. This introduces, however, a number of difficulties due to the scanning process, such as lower frame rates due to the serial acquisition process. Here we show that by introducing spatiotemporal pulse shaping techniques to multiphoton microscopy it is possible to obtain full-frame depth resolved imaging completely without scanning. Our method relies on temporal focusing of the illumination pulse. The pulsed excitation field is compressed as it propagates through the sample, reaching its shortest duration at the focal plane, before stretching again beyond it. This method is applied to obtain depth-resolved two-photon excitation fluorescence (TPEF) images of drosophila egg-chambers with nearly 105 effective pixels using a standard Ti:Sapphire laser oscillator.
UR - http://www.scopus.com/inward/record.url?scp=17444392148&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=17444392148&partnerID=8YFLogxK
U2 - 10.1364/OPEX.13.001468
DO - 10.1364/OPEX.13.001468
M3 - Article
VL - 13
SP - 1468
EP - 1476
JO - Optics Express
JF - Optics Express
SN - 1094-4087
IS - 5
ER -