Selective Differentiation of Neural Progenitor Cells by High-Epitope Density Nanofibers

Gabriel A. Silva, Catherine Czeisler, Krista L. Niece, Elia Beniash, Daniel A. Harrington, John A. Kessler, Samuel I. Stupp

Research output: Contribution to journalArticle

1720 Citations (Scopus)

Abstract

Neural progenitor cells were encapsulated in vitro within a three-dimensional network of nanofibers formed by self-assembly of peptide amphiphile molecules. The self-assembly is triggered by mixing cell suspensions in media with dilute aqueous solutions of the molecules, and cells survive the growth of the nanofibers around them. These nanofibers were designed to present to cells the neurite-promoting laminin epitope IKVAV at nearly van der Waals density. Relative to laminin or soluble peptide, the artificial nanofiber scaffold induced very rapid differentiation of cells into neurons, while discouraging the development of astrocytes. This rapid selective differentiation is linked to the amplification of bioactive epitope presentation to cells by the nanofibers.

Original languageEnglish
Pages (from-to)1352-1355
Number of pages4
JournalScience
Volume303
Issue number5662
DOIs
Publication statusPublished - Feb 27 2004

ASJC Scopus subject areas

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    Silva, G. A., Czeisler, C., Niece, K. L., Beniash, E., Harrington, D. A., Kessler, J. A., & Stupp, S. I. (2004). Selective Differentiation of Neural Progenitor Cells by High-Epitope Density Nanofibers. Science, 303(5662), 1352-1355. https://doi.org/10.1126/science.1093783