Abstract
An important goal of nanotechnology is to assemble multiple molecules while controlling the spacing between them. Of particular interest is the phenomenon of multivalency, which is characterized by simultaneous binding of multiple ligands on one biological entity to multiple receptors on another. Various approaches have been developed to engineer multivalency by linking multiple ligands together. However, the effects of well-controlled inter-ligand distances on multivalency are less well understood. Recent progress in self-assembling DNA nanostructures with spatial and sequence addressability has made deterministic positioning of different molecular species possible. Here we show that distance-dependent multivalent binding effects can be systematically investigated by incorporating multiple-affinity ligands into DNA nanostructures with precise nanometre spatial control. Using atomic force microscopy, we demonstrate direct visualization of high-affinity bivalent ligands being used as pincers to capture and display protein molecules on a nanoarray. These results illustrate the potential of using designer DNA nanoscaffolds to engineer more complex and interactive biomolecular networks.
| Original language | English |
|---|---|
| Pages (from-to) | 418-422 |
| Number of pages | 5 |
| Journal | Nature Nanotechnology |
| Volume | 3 |
| Issue number | 7 |
| DOIs | |
| Publication status | Published - Jul 2008 |
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ASJC Scopus subject areas
- Bioengineering
- Biomedical Engineering
- Materials Science(all)
- Electrical and Electronic Engineering
- Condensed Matter Physics
- Atomic and Molecular Physics, and Optics
Cite this
Self-assembled DNA nanostructures for distance-dependent multivalent ligand-protein binding. / Rinker, Sherri; Ke, Yonggang; Liu, Yan; Chhabra, Rahul; Yan, Hao.
In: Nature Nanotechnology, Vol. 3, No. 7, 07.2008, p. 418-422.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Self-assembled DNA nanostructures for distance-dependent multivalent ligand-protein binding
AU - Rinker, Sherri
AU - Ke, Yonggang
AU - Liu, Yan
AU - Chhabra, Rahul
AU - Yan, Hao
PY - 2008/7
Y1 - 2008/7
N2 - An important goal of nanotechnology is to assemble multiple molecules while controlling the spacing between them. Of particular interest is the phenomenon of multivalency, which is characterized by simultaneous binding of multiple ligands on one biological entity to multiple receptors on another. Various approaches have been developed to engineer multivalency by linking multiple ligands together. However, the effects of well-controlled inter-ligand distances on multivalency are less well understood. Recent progress in self-assembling DNA nanostructures with spatial and sequence addressability has made deterministic positioning of different molecular species possible. Here we show that distance-dependent multivalent binding effects can be systematically investigated by incorporating multiple-affinity ligands into DNA nanostructures with precise nanometre spatial control. Using atomic force microscopy, we demonstrate direct visualization of high-affinity bivalent ligands being used as pincers to capture and display protein molecules on a nanoarray. These results illustrate the potential of using designer DNA nanoscaffolds to engineer more complex and interactive biomolecular networks.
AB - An important goal of nanotechnology is to assemble multiple molecules while controlling the spacing between them. Of particular interest is the phenomenon of multivalency, which is characterized by simultaneous binding of multiple ligands on one biological entity to multiple receptors on another. Various approaches have been developed to engineer multivalency by linking multiple ligands together. However, the effects of well-controlled inter-ligand distances on multivalency are less well understood. Recent progress in self-assembling DNA nanostructures with spatial and sequence addressability has made deterministic positioning of different molecular species possible. Here we show that distance-dependent multivalent binding effects can be systematically investigated by incorporating multiple-affinity ligands into DNA nanostructures with precise nanometre spatial control. Using atomic force microscopy, we demonstrate direct visualization of high-affinity bivalent ligands being used as pincers to capture and display protein molecules on a nanoarray. These results illustrate the potential of using designer DNA nanoscaffolds to engineer more complex and interactive biomolecular networks.
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UR - http://www.scopus.com/inward/citedby.url?scp=46749136342&partnerID=8YFLogxK
U2 - 10.1038/nnano.2008.164
DO - 10.1038/nnano.2008.164
M3 - Article
C2 - 18654566
AN - SCOPUS:46749136342
VL - 3
SP - 418
EP - 422
JO - Nature Nanotechnology
JF - Nature Nanotechnology
SN - 1748-3387
IS - 7
ER -