TY - JOUR
T1 - Self-assembled DNA nanostructures for distance-dependent multivalent ligand-protein binding
AU - Rinker, Sherri
AU - Ke, Yonggang
AU - Liu, Yan
AU - Chhabra, Rahul
AU - Yan, Hao
N1 - Funding Information:
This work was derived from a discussion of the synthetic antibody project with S.A. Johnston at the Biodesign Institute, Arizona State University. We also thank S. Lindsay, C. Diehnelt and Dong-Kyun Seo for helpful discussions. S.R. was partly supported by the Technology and Research Initiative Fund from Arizona State University to S.A. Johnston. This work was partly supported by grants from the National Science Foundation, the National Institute of Health, the Air Force Office of Scientific Research and the Office of Naval Research to Hao Yan and TRIF funds from Arizona State University to Hao Yan and Yan Liu.
PY - 2008/7
Y1 - 2008/7
N2 - An important goal of nanotechnology is to assemble multiple molecules while controlling the spacing between them. Of particular interest is the phenomenon of multivalency, which is characterized by simultaneous binding of multiple ligands on one biological entity to multiple receptors on another. Various approaches have been developed to engineer multivalency by linking multiple ligands together. However, the effects of well-controlled inter-ligand distances on multivalency are less well understood. Recent progress in self-assembling DNA nanostructures with spatial and sequence addressability has made deterministic positioning of different molecular species possible. Here we show that distance-dependent multivalent binding effects can be systematically investigated by incorporating multiple-affinity ligands into DNA nanostructures with precise nanometre spatial control. Using atomic force microscopy, we demonstrate direct visualization of high-affinity bivalent ligands being used as pincers to capture and display protein molecules on a nanoarray. These results illustrate the potential of using designer DNA nanoscaffolds to engineer more complex and interactive biomolecular networks.
AB - An important goal of nanotechnology is to assemble multiple molecules while controlling the spacing between them. Of particular interest is the phenomenon of multivalency, which is characterized by simultaneous binding of multiple ligands on one biological entity to multiple receptors on another. Various approaches have been developed to engineer multivalency by linking multiple ligands together. However, the effects of well-controlled inter-ligand distances on multivalency are less well understood. Recent progress in self-assembling DNA nanostructures with spatial and sequence addressability has made deterministic positioning of different molecular species possible. Here we show that distance-dependent multivalent binding effects can be systematically investigated by incorporating multiple-affinity ligands into DNA nanostructures with precise nanometre spatial control. Using atomic force microscopy, we demonstrate direct visualization of high-affinity bivalent ligands being used as pincers to capture and display protein molecules on a nanoarray. These results illustrate the potential of using designer DNA nanoscaffolds to engineer more complex and interactive biomolecular networks.
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U2 - 10.1038/nnano.2008.164
DO - 10.1038/nnano.2008.164
M3 - Article
C2 - 18654566
AN - SCOPUS:46749136342
VL - 3
SP - 418
EP - 422
JO - Nature Nanotechnology
JF - Nature Nanotechnology
SN - 1748-3387
IS - 7
ER -