Self-assembled water-soluble nucleic acid probe tiles for label-free RNA hybridization assays

Yonggang Ke, Stuart Lindsay, Yung Chang, Yan Liu, Hao Yan

Research output: Contribution to journalArticle

348 Citations (Scopus)

Abstract

The DNA origami method, in which long, single-stranded DNA segments are folded into shapes by short staple segments, was used to create nucleic acid probe tiles that are molecular analogs of macroscopic DNA chips. One hundred trillion probe tiles were fabricated in one step and bear pairs of 20-nucleotide-long single-stranded DNA segments that act as probe sequences. These tiles can hybridize to their targets in solution and, after adsorption onto mica surfaces, can be examined by atomic force microscopy in order to quantify binding events, because the probe segments greatly increase in stiffness upon hybridization. The nucleic acid probe tiles have been used to study position-dependent hybridization on the nanoscale and have also been used for label-free detection of RNA.

Original languageEnglish
Pages (from-to)180-183
Number of pages4
JournalScience
Volume319
Issue number5860
DOIs
Publication statusPublished - Jan 11 2008

ASJC Scopus subject areas

  • General

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