Self-assembling nanofibers inhibit glial scar formation and promote axon elongation after spinal cord injury

Vicki M. Tysseling-Mattiace, Vibhu Sahni, Krista L. Niece, Derin Birch, Catherine Czeisler, Michael G. Fehlings, Samuel I Stupp, John A. Kessler

Research output: Contribution to journalArticle

475 Citations (Scopus)

Abstract

Peptide amphiphile (PA) molecules that self-assemble in vivo into supramolecular nanofibers were used as a therapy in a mouse model of spinal cord injury (SCI). Because self-assembly of these molecules is triggered by the ionic strength of the in vivo environment, nanoscale structures can be created within the extracellular spaces of the spinal cord by simply injecting a liquid. The molecules are designed to form cylindrical nanofibers that display to cells in the spinal cord the laminin epitope IKVAV at nearly van der Waals density. IKVAV PA nanofibers are known to inhibit glial differentiation of cultured neural stem cells and to promote neurite outgrowth from cultured neurons. In this work, in vivo treatment with the PA after SCI reduced astrogliosis, reduced cell death, and increased the number of oligodendroglia at the site of injury. Furthermore, the nanofibers promoted regeneration of both descending motor fibers and ascending sensory fibers through the lesion site. Treatment with the PA also resulted in significant behavioral improvement. These observations demonstrate that it is possible to inhibit glial scar formation and to facilitate regeneration after SCI using bioactive three-dimensional nanostructures displaying high densities of neuroactive epitopes on their surfaces.

Original languageEnglish
Pages (from-to)3814-3823
Number of pages10
JournalJournal of Neuroscience
Volume28
Issue number14
DOIs
Publication statusPublished - Apr 2 2008

Fingerprint

Nanofibers
isoleucyl-lysyl-valyl-alanyl-valine
Spinal Cord Injuries
Neuroglia
Cicatrix
Axons
Peptides
Epitopes
Regeneration
Spinal Cord
Neural Stem Cells
Nanostructures
Oligodendroglia
Extracellular Space
Laminin
Osmolar Concentration
Cell Death
Neurons
Wounds and Injuries

Keywords

  • Extracellular matrix
  • Functional recovery
  • Gliosis
  • Nanotechnology
  • Regeneration
  • Spinal cord injury

ASJC Scopus subject areas

  • Neuroscience(all)
  • Medicine(all)

Cite this

Tysseling-Mattiace, V. M., Sahni, V., Niece, K. L., Birch, D., Czeisler, C., Fehlings, M. G., ... Kessler, J. A. (2008). Self-assembling nanofibers inhibit glial scar formation and promote axon elongation after spinal cord injury. Journal of Neuroscience, 28(14), 3814-3823. https://doi.org/10.1523/JNEUROSCI.0143-08.2008

Self-assembling nanofibers inhibit glial scar formation and promote axon elongation after spinal cord injury. / Tysseling-Mattiace, Vicki M.; Sahni, Vibhu; Niece, Krista L.; Birch, Derin; Czeisler, Catherine; Fehlings, Michael G.; Stupp, Samuel I; Kessler, John A.

In: Journal of Neuroscience, Vol. 28, No. 14, 02.04.2008, p. 3814-3823.

Research output: Contribution to journalArticle

Tysseling-Mattiace, VM, Sahni, V, Niece, KL, Birch, D, Czeisler, C, Fehlings, MG, Stupp, SI & Kessler, JA 2008, 'Self-assembling nanofibers inhibit glial scar formation and promote axon elongation after spinal cord injury', Journal of Neuroscience, vol. 28, no. 14, pp. 3814-3823. https://doi.org/10.1523/JNEUROSCI.0143-08.2008
Tysseling-Mattiace, Vicki M. ; Sahni, Vibhu ; Niece, Krista L. ; Birch, Derin ; Czeisler, Catherine ; Fehlings, Michael G. ; Stupp, Samuel I ; Kessler, John A. / Self-assembling nanofibers inhibit glial scar formation and promote axon elongation after spinal cord injury. In: Journal of Neuroscience. 2008 ; Vol. 28, No. 14. pp. 3814-3823.
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