Self-assembling peptide amphiphile nanofiber matrices for cell entrapment

Elia Beniash, Jeffery D. Hartgerink, Hannah Storrie, John C. Stendahl, Samuel I Stupp

Research output: Contribution to journalArticle

220 Citations (Scopus)

Abstract

We have developed a class of peptide amphiphile (PA) molecules that self-assemble into three-dimensional nanofiber networks under physiological conditions in the presence of polyvalent metal ions. The assembly can be triggered by adding PA solutions to cell culture media or other synthetic physiological fluids containing polyvalent metal ions. When the fluids contain suspended cells, PA self-assembly entraps cells in the nanofibrillar matrix, and the cells survive in culture for at least three weeks. We also show that entrapment does not arrest cell proliferation and motility. Biochemical and ultrastructural analysis by electron microscopy indicate that entrapped cells internalize the nanofibers and possibly utilize PA molecules in their metabolic pathways. These results demonstrate that PA nanofibrillar matrices have the potential to be used for cell transplantation or other tissue engineering applications.

Original languageEnglish
Pages (from-to)387-397
Number of pages11
JournalActa Biomaterialia
Volume1
Issue number4
DOIs
Publication statusPublished - Jul 2005

Fingerprint

Nanofibers
Amphiphiles
Peptides
Metal ions
Metals
Ions
Transplantation (surgical)
Molecules
Fluids
Cell Transplantation
Cell proliferation
Tissue Engineering
Metabolic Networks and Pathways
Tissue engineering
Cell culture
Self assembly
Electron microscopy
Cell Movement
Culture Media
Electron Microscopy

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering
  • Biotechnology
  • Biochemistry
  • Molecular Biology

Cite this

Self-assembling peptide amphiphile nanofiber matrices for cell entrapment. / Beniash, Elia; Hartgerink, Jeffery D.; Storrie, Hannah; Stendahl, John C.; Stupp, Samuel I.

In: Acta Biomaterialia, Vol. 1, No. 4, 07.2005, p. 387-397.

Research output: Contribution to journalArticle

Beniash, Elia ; Hartgerink, Jeffery D. ; Storrie, Hannah ; Stendahl, John C. ; Stupp, Samuel I. / Self-assembling peptide amphiphile nanofiber matrices for cell entrapment. In: Acta Biomaterialia. 2005 ; Vol. 1, No. 4. pp. 387-397.
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