Self-assembly combining two bioactive peptide-amphiphile molecules into nanofibers by electrostatic attraction

Krista L. Niece, Jeffrey D. Hartgerink, Jack J J M Donners, Samuel I. Stupp

Research output: Contribution to journalArticle

354 Citations (Scopus)

Abstract

We describe a new approach to peptide-amphiphile (PA) nanofiber preparation that allows PAs with different bioactive amino acid sequences to be combined into a single fiber. Oppositely charged PAs are synthesized separately and then mixed to produce gels of nanofiber networks at physiological pH. Transmission electron microscopy reveals the formation of fibers approximately 7 nm in diameter and several micrometers long in these dimeric systems. On the basis of NMR and microscopy, we suggest that these nanofibers are cylindrical micelles of mixed composition, formed due to electrostatic attraction between the oppositely charged PAs. This strategy for self-assembly may be useful in cell therapies that can be implemented without invasive surgery or in in vitro tissue engineering.

Original languageEnglish
Pages (from-to)7146-7147
Number of pages2
JournalJournal of the American Chemical Society
Volume125
Issue number24
DOIs
Publication statusPublished - Jun 18 2003

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Nanofibers
Amphiphiles
Static Electricity
Self assembly
Peptides
Electrostatics
Molecules
Fibers
Micelles
Tissue Engineering
Cell- and Tissue-Based Therapy
Transmission Electron Microscopy
Tissue engineering
Surgery
Amino acids
Microscopy
Amino Acid Sequence
Microscopic examination
Gels
Nuclear magnetic resonance

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Self-assembly combining two bioactive peptide-amphiphile molecules into nanofibers by electrostatic attraction. / Niece, Krista L.; Hartgerink, Jeffrey D.; Donners, Jack J J M; Stupp, Samuel I.

In: Journal of the American Chemical Society, Vol. 125, No. 24, 18.06.2003, p. 7146-7147.

Research output: Contribution to journalArticle

Niece, Krista L. ; Hartgerink, Jeffrey D. ; Donners, Jack J J M ; Stupp, Samuel I. / Self-assembly combining two bioactive peptide-amphiphile molecules into nanofibers by electrostatic attraction. In: Journal of the American Chemical Society. 2003 ; Vol. 125, No. 24. pp. 7146-7147.
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