Separation, Immobilization, and Biocatalytic Utilization of Proteins by a Supramolecular Membrane

Elisha Krieg, Shira Albeck, Haim Weissman, Eyal Shimoni, Boris Rybtchinski

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Membrane separation of biomolecules and their application in biocatalysis is becoming increasingly important for biotechnology, demanding the development of new biocompatible materials with novel properties. In the present study, an entirely noncovalent water-based material is used as a membrane for size-selective separation, immobilization, and biocatalytic utilization of proteins. The membrane shows stable performance under physiological conditions, allowing filtration of protein mixtures with a 150 kDa molecular weight cutoff (∼8 nm hydrodynamic diameter cutoff). Due to the biocompatibility of the membrane, filtered proteins stay functionally active and retained proteins can be partially recovered. Upon filtration, large enzymes become immobilized within the membrane. They exhibit stable activity when subjected to a constant flux of substrates for prolonged periods of time, which can be used to carry out heterogeneous biocatalysis. The noncovalent membrane material can be easily disassembled, purified, reassembled, and reused, showing reproducible performance after recycling. The robustness, recyclability, versatility, and biocompatibility of the supramolecular membrane may open new avenues for manipulating biological systems.

Original languageEnglish
Article numbere63188
JournalPLoS One
Volume8
Issue number5
DOIs
Publication statusPublished - May 10 2013

Fingerprint

Immobilization
biocompatibility
Membranes
Biocatalysis
immobilized enzymes
Proteins
biocompatible materials
proteins
Biocompatibility
membrane proteins
biotechnology
hydrodynamics
recycling
molecular weight
Immobilized Enzymes
Recycling
Biocompatible Materials
Biomolecules
Biological systems
Hydrodynamics

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Separation, Immobilization, and Biocatalytic Utilization of Proteins by a Supramolecular Membrane. / Krieg, Elisha; Albeck, Shira; Weissman, Haim; Shimoni, Eyal; Rybtchinski, Boris.

In: PLoS One, Vol. 8, No. 5, e63188, 10.05.2013.

Research output: Contribution to journalArticle

Krieg, Elisha ; Albeck, Shira ; Weissman, Haim ; Shimoni, Eyal ; Rybtchinski, Boris. / Separation, Immobilization, and Biocatalytic Utilization of Proteins by a Supramolecular Membrane. In: PLoS One. 2013 ; Vol. 8, No. 5.
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