Serial femtosecond X-ray diffraction of enveloped virus microcrystals

Robert M. Lawrence, Chelsie E. Conrad, Nadia A. Zatsepin, Thomas D. Grant, Haiguang Liu, Daniel James, Garrett Nelson, Ganesh Subramanian, Andrew Aquila, Mark S. Hunter, Mengning Liang, Sébastien Boutet, Jesse Coe, John C.H. Spence, Uwe Weierstall, Wei Liu, Petra Fromme, Vadim Cherezov, Brenda G. Hogue

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Serial femtosecond crystallography (SFX) using X-ray free-electron lasers has produced high-resolution, room temperature, time-resolved protein structures. We report preliminary SFX of Sindbis virus, an enveloped icosahedral RNA virus with ~700A ° diameter. Microcrystals delivered in viscous agarose medium diffracted to ~40A ° resolution. Small-angle diffuse X-ray scattering overlaid Bragg peaks and analysis suggests this results from molecular transforms of individual particles. Viral proteins undergo structural changes during entry and infection, which could, in principle, be studied with SFX. This is an important step toward determining room temperature structures from virus microcrystals that may enable time-resolved studies of enveloped viruses.

Original languageEnglish
Article number041720
JournalStructural Dynamics
Volume2
Issue number4
DOIs
Publication statusPublished - Jul 1 2015

ASJC Scopus subject areas

  • Radiation
  • Instrumentation
  • Condensed Matter Physics
  • Spectroscopy

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    Lawrence, R. M., Conrad, C. E., Zatsepin, N. A., Grant, T. D., Liu, H., James, D., Nelson, G., Subramanian, G., Aquila, A., Hunter, M. S., Liang, M., Boutet, S., Coe, J., Spence, J. C. H., Weierstall, U., Liu, W., Fromme, P., Cherezov, V., & Hogue, B. G. (2015). Serial femtosecond X-ray diffraction of enveloped virus microcrystals. Structural Dynamics, 2(4), [041720]. https://doi.org/10.1063/1.4929410