SERS aptatags

New responsive metallic nanostructures for heterogeneous protein detection by surface enhanced raman spectroscopy

Laura Fabris, Mark Dante, Thuc Quyen Nguyen, Jeffrey B H Tok, Guillermo C. Bazan

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

We report here "aptatags," which consist of aptamer-modified silver nanoparticles (NPs) held together by an optical reporter. It is possible to use these materials to design a heterogeneous method for protein identification that takes advantage of the Raman signal enhancement by metallic nanostructures and the recognition capabilities of aptamers. Aptatags are formed by linking silver NPs with an organic dithiol molecule, followed by surface modification with thiolated single-stranded DNA (ssDNA) corresponding to the sequence of the aptamer probe. The sensing surface involves a silver layer containing the thiolated capturing aptamer and mercaptohexadecanoic acid to minimize nonspecific binding. The overall process provides excellent selectivity and sensitivity. Detailed characterization of the sensing surface by SERS maps and atomic force microscopy was carried out to understand how structural features lead to signal generation.

Original languageEnglish
Pages (from-to)2518-2525
Number of pages8
JournalAdvanced Functional Materials
Volume18
Issue number17
DOIs
Publication statusPublished - Sep 10 2008

Fingerprint

Silver
Raman spectroscopy
Nanostructures
Membrane Proteins
silver
proteins
Proteins
Nanoparticles
nanoparticles
Single-Stranded DNA
thiols
Surface treatment
Atomic force microscopy
DNA
deoxyribonucleic acid
selectivity
atomic force microscopy
acids
Molecules
Acids

ASJC Scopus subject areas

  • Biomaterials
  • Electrochemistry
  • Condensed Matter Physics
  • Electronic, Optical and Magnetic Materials

Cite this

SERS aptatags : New responsive metallic nanostructures for heterogeneous protein detection by surface enhanced raman spectroscopy. / Fabris, Laura; Dante, Mark; Nguyen, Thuc Quyen; Tok, Jeffrey B H; Bazan, Guillermo C.

In: Advanced Functional Materials, Vol. 18, No. 17, 10.09.2008, p. 2518-2525.

Research output: Contribution to journalArticle

Fabris, Laura ; Dante, Mark ; Nguyen, Thuc Quyen ; Tok, Jeffrey B H ; Bazan, Guillermo C. / SERS aptatags : New responsive metallic nanostructures for heterogeneous protein detection by surface enhanced raman spectroscopy. In: Advanced Functional Materials. 2008 ; Vol. 18, No. 17. pp. 2518-2525.
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