Abstract
This Communication demonstrates how selectively functionalized, patterned microscale wells can be used as small-volume (fL) crystallization vessels to grow protein crystals. This surface-patterned approach enables proteins to be crystallized with well-controlled shapes and uniform sizes from 3 μm down to 600 nm. Three different proteins - lysozyme, thaumatin, and glucose isomerase - were crystallized as model systems to demonstrate the generality of this technique. Different polymorphs were formed simply by controlling the evaporation conditions.
Original language | English |
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Pages (from-to) | 2142-2143 |
Number of pages | 2 |
Journal | Journal of the American Chemical Society |
Volume | 130 |
Issue number | 7 |
DOIs | |
Publication status | Published - Feb 20 2008 |
ASJC Scopus subject areas
- Catalysis
- Chemistry(all)
- Biochemistry
- Colloid and Surface Chemistry