Signal amplification on a DNA-tile-based biosensor with enhanced sensitivity

Chenxiang Lin, Jeanette K. Nangreave, Zhe Li, Yan Liu, Hao Yan

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Aims: Herein, we report our work to improve the detection sensitivity of a DNA-tile-based and self-assembled biosensing platform. This was achieved using hybridization chain reaction (HCR) as a signal amplifier on a water-soluble self-assembled DNA nanoarray carrying detection probes. Materials & methods: The fluorescence enhancement on the addition of specific detection targets was observed directly by confocal fluorescence microscopy. Results & discussion: The versatility of the system was demonstrated by successful detection of SARS viral DNA and ATR Improvement of sensitivity by two orders of magnitude was achieved for both targets, compared with our previously reported system without signal amplification. Conclusion: In summary, this work provides proof-of-concept that HCR can occur efficiently on DNA-tile-based nanoarrays, thus facilitating more sensitive detection. The merging of HCR with combinatorial encoding systems to realize highly sensitive and multiplexed biosensing may provide new tools for nanomedical applications.

Original languageEnglish
Pages (from-to)521-528
Number of pages8
JournalNanomedicine
Volume3
Issue number4
DOIs
Publication statusPublished - Aug 2008

Fingerprint

Biosensing Techniques
Tile
Biosensors
Amplification
amplification
DNA
Signal systems
Confocal microscopy
Fluorescence microscopy
Viral DNA
Target tracking
Fluorescence Microscopy
Merging
Confocal Microscopy
fluorescence
severe acute respiratory syndrome
Fluorescence
water
Water
microscopy

Keywords

  • Biosensing
  • DNA nanotechnology
  • Hybridization chain reaction
  • Self-assembly
  • Signal amplification

ASJC Scopus subject areas

  • Materials Science(all)
  • Bioengineering
  • Biomedical Engineering
  • Medicine (miscellaneous)
  • Development

Cite this

Signal amplification on a DNA-tile-based biosensor with enhanced sensitivity. / Lin, Chenxiang; Nangreave, Jeanette K.; Li, Zhe; Liu, Yan; Yan, Hao.

In: Nanomedicine, Vol. 3, No. 4, 08.2008, p. 521-528.

Research output: Contribution to journalArticle

Lin, Chenxiang ; Nangreave, Jeanette K. ; Li, Zhe ; Liu, Yan ; Yan, Hao. / Signal amplification on a DNA-tile-based biosensor with enhanced sensitivity. In: Nanomedicine. 2008 ; Vol. 3, No. 4. pp. 521-528.
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