Abstract
Self-folding of an information-carrying polymer into a defined structure is foundational to biology and offers attractive potential as a synthetic strategy. Although multicomponent self-assembly has produced complex synthetic nanostructures, unimolecular folding has seen limited progress.We describe a framework to design and synthesize a single DNA or RNA strand to self-fold into a complex yet unknotted structure that approximates an arbitrary user-prescribed shape. We experimentally construct diverse multikilobase single-stranded structures, including a ∼10, 000-nucleotide (nt) DNA structure and a ∼6000-nt RNA structure. We demonstrate facile replication of the strand in vitro and in living cells. The work here thus establishes unimolecular folding as a general strategy for constructing complex and replicable nucleic acid nanostructures, and expands the design space and material scalability for bottom-up nanotechnology.
| Original language | English |
|---|---|
| Article number | aao2648 |
| Journal | Science |
| Volume | 358 |
| Issue number | 6369 |
| DOIs | |
| Publication status | Published - Dec 15 2017 |
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ASJC Scopus subject areas
- General
Cite this
Single-stranded DNA and RNA origami. / Han, Dongran; Qi, Xiaodong; Myhrvold, Cameron; Wang, Bei; Dai, Mingjie; Jiang, Shuoxing; Bates, Maxwell; Liu, Yan; An, Byoungkwon; Zhang, Fei; Yan, Hao; Yin, Peng.
In: Science, Vol. 358, No. 6369, aao2648, 15.12.2017.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Single-stranded DNA and RNA origami
AU - Han, Dongran
AU - Qi, Xiaodong
AU - Myhrvold, Cameron
AU - Wang, Bei
AU - Dai, Mingjie
AU - Jiang, Shuoxing
AU - Bates, Maxwell
AU - Liu, Yan
AU - An, Byoungkwon
AU - Zhang, Fei
AU - Yan, Hao
AU - Yin, Peng
PY - 2017/12/15
Y1 - 2017/12/15
N2 - Self-folding of an information-carrying polymer into a defined structure is foundational to biology and offers attractive potential as a synthetic strategy. Although multicomponent self-assembly has produced complex synthetic nanostructures, unimolecular folding has seen limited progress.We describe a framework to design and synthesize a single DNA or RNA strand to self-fold into a complex yet unknotted structure that approximates an arbitrary user-prescribed shape. We experimentally construct diverse multikilobase single-stranded structures, including a ∼10, 000-nucleotide (nt) DNA structure and a ∼6000-nt RNA structure. We demonstrate facile replication of the strand in vitro and in living cells. The work here thus establishes unimolecular folding as a general strategy for constructing complex and replicable nucleic acid nanostructures, and expands the design space and material scalability for bottom-up nanotechnology.
AB - Self-folding of an information-carrying polymer into a defined structure is foundational to biology and offers attractive potential as a synthetic strategy. Although multicomponent self-assembly has produced complex synthetic nanostructures, unimolecular folding has seen limited progress.We describe a framework to design and synthesize a single DNA or RNA strand to self-fold into a complex yet unknotted structure that approximates an arbitrary user-prescribed shape. We experimentally construct diverse multikilobase single-stranded structures, including a ∼10, 000-nucleotide (nt) DNA structure and a ∼6000-nt RNA structure. We demonstrate facile replication of the strand in vitro and in living cells. The work here thus establishes unimolecular folding as a general strategy for constructing complex and replicable nucleic acid nanostructures, and expands the design space and material scalability for bottom-up nanotechnology.
UR - http://www.scopus.com/inward/record.url?scp=85040195228&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85040195228&partnerID=8YFLogxK
U2 - 10.1126/science.aao2648
DO - 10.1126/science.aao2648
M3 - Article
C2 - 29242318
AN - SCOPUS:85040195228
VL - 358
JO - Science
JF - Science
SN - 0036-8075
IS - 6369
M1 - aao2648
ER -