Size based nanoparticle separation using dielectrophoretic focusing for femtosecond nanocrystallography of membrane proteins

Bahige Abdallah, Tzu Chiao Chao, Petra Fromme, Alexandra Ros

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Citations (Scopus)

Abstract

We propose a method to separate photosystem I crystals based on size using a combination of dielectrophoresis (DEP) and electrokinesis (EK) within a microfluidic device. In this work, a model system utilizing polystyrene beads of two sizes is employed to observe the effects of DEP and EK on particles as they pass through a microsorter via electroosmosis. Particle counting and fluorescence intensity measurements are used for quantitative analysis of experimental data. For comparison, numerical simulations were performed for further confirmation that the proposed device is capable of sorting particles based on their size. Our experimental and theoretical results are in agreement and show a high degree of sorting efficiency between both particle types making this a promising solution for protein crystal sorting.

Original languageEnglish
Title of host publicationProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
PublisherChemical and Biological Microsystems Society
Pages458-460
Number of pages3
ISBN (Print)9780979806452
Publication statusPublished - 2012
Event16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 - Okinawa, Japan
Duration: Oct 28 2012Nov 1 2012

Other

Other16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
CountryJapan
CityOkinawa
Period10/28/1211/1/12

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Keywords

  • Crystallography
  • Dielectrophoresis
  • Microfluidics
  • Nanoparticle
  • Separation

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

Cite this

Abdallah, B., Chao, T. C., Fromme, P., & Ros, A. (2012). Size based nanoparticle separation using dielectrophoretic focusing for femtosecond nanocrystallography of membrane proteins. In Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 (pp. 458-460). Chemical and Biological Microsystems Society.