Smaller CpG-Conjugated Gold Nanoconstructs Achieve Higher Targeting Specificity of Immune Activation

Jun Yue, Roger M. Pallares, Lisa E. Cole, Emma E. Coughlin, Chad A. Mirkin, Andrew Lee, Teri W. Odom

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

This study describes a side-by-side comparison of the in vitro immunostimulatory activity of cytosine-phosphate-guanine (CpG)-conjugated gold nanoparticles. Three different gold nanoparticle cores (13 nm spheres, 50 nm spheres, and 40 nm stars) with the same CpG surface density were investigated for toll-like receptor 9 activation. For this parameter set, 13 nm spheres displayed significantly higher specificity for targeting immune receptors and larger nanoparticles (50 nm spheres and 40 nm stars) showed higher cellular uptake and higher immune activation because of off-target effects. Changes in nanoparticle size and presentation of activating ligands affect construct-induced immune responses at different levels, and care must be taken when considering practical and global design rules for CpG delivery.

Original languageEnglish
Pages (from-to)21920-21926
Number of pages7
JournalACS Applied Materials and Interfaces
Volume10
Issue number26
DOIs
Publication statusPublished - Jul 5 2018

Keywords

  • CpG
  • cellular uptake
  • gold nanoparticles
  • immunostimulatory activity
  • targeting

ASJC Scopus subject areas

  • Materials Science(all)

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