TY - JOUR
T1 - SOFISM
T2 - Super-resolution optical fluctuation image scanning microscopy
AU - Sroda, Aleksandra
AU - Makowski, Adrian
AU - Tenne, Ron
AU - Rossman, Uri
AU - Lubin, Gur
AU - Oron, Dan
AU - Lapkiewicz, Radek
N1 - Funding Information:
Funding. European Research Council (ColloQuantO); Crown Photonics Center; Minerva Foundation; KLA-TENCOR Corporation; Narodowe Centrum Nauki (2015/17/D/ST2/ 03471); Ministerstwo Nauki i Szkolnictwa Wyższego; Foundation for Polish Science under the FIRST TEAM project ‘Spatiotemporal photon correlation measurements for quantum metrology and super-resolution microscopy’ co-financed by the European Union under the European Regional Development Fund (POIR.04.04.00-00-3004/17-00).
PY - 2020/10
Y1 - 2020/10
N2 - Super-resolution optical microscopy is a rapidly evolving scientific field dedicated to imaging sub-wavelength-sized objects, leaving its mark in multiple branches of biology and technology. While several super-resolution optical microscopy methods have become a common tool in life science imaging, new methods, supported by cutting-edge technology, continue to emerge. One rather recent addition to the super-resolution toolbox, image scanning microscopy (ISM), achieves up to twofold lateral resolution enhancement in a robust and straightforward manner. To further enhance ISM's resolution in all three dimensions, we present and experimentally demonstrate here super-resolution optical fluctuation ISM (SOFISM). Measuring the fluorescence fluctuation contrast in an ISM architecture, we obtain images with a ×2.5 lateral resolution beyond the diffraction limit along with an enhanced axial resolution for a fixed cell sample labeled with commercially available quantum dots. The inherent temporal averaging of the ISM technique enables image acquisition of the fluctuation correlation contrast within millisecond-scale pixel dwell times. SOFISM can therefore offer a robust path to achieve high-resolution images within a slightly modified confocal microscope, using standard fluorescent labels and reasonable acquisition times.
AB - Super-resolution optical microscopy is a rapidly evolving scientific field dedicated to imaging sub-wavelength-sized objects, leaving its mark in multiple branches of biology and technology. While several super-resolution optical microscopy methods have become a common tool in life science imaging, new methods, supported by cutting-edge technology, continue to emerge. One rather recent addition to the super-resolution toolbox, image scanning microscopy (ISM), achieves up to twofold lateral resolution enhancement in a robust and straightforward manner. To further enhance ISM's resolution in all three dimensions, we present and experimentally demonstrate here super-resolution optical fluctuation ISM (SOFISM). Measuring the fluorescence fluctuation contrast in an ISM architecture, we obtain images with a ×2.5 lateral resolution beyond the diffraction limit along with an enhanced axial resolution for a fixed cell sample labeled with commercially available quantum dots. The inherent temporal averaging of the ISM technique enables image acquisition of the fluctuation correlation contrast within millisecond-scale pixel dwell times. SOFISM can therefore offer a robust path to achieve high-resolution images within a slightly modified confocal microscope, using standard fluorescent labels and reasonable acquisition times.
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U2 - 10.1364/OPTICA.399600
DO - 10.1364/OPTICA.399600
M3 - Article
AN - SCOPUS:85092289296
VL - 7
SP - 1308
EP - 1316
JO - Optica
JF - Optica
SN - 2334-2536
IS - 10
ER -