Abstract
The ability to perform optical sectioning is one of the great advantages of laser-scanning microscopy. This introduces, however, a number of difficulties due to the scanning process, such as lower frame rates due to the serial acquisition process. Here we show that by introducing spatiotemporal pulse shaping techniques to multiphoton microscopy it is possible to obtain full-frame depth resolved imaging completely without scanning. Our method relies on temporal focusing of the illumination pulse. The pulsed excitation field is compressed as it propagates through the sample, reaching its shortest duration at the focal plane, before stretching again beyond it. Combining temporal focusing with line-scanning microscopy results in an enhanced depth resolution, equivalent to that achieved by point scanning. Both the scanningless and the line-scanning techniques are applied to obtain depth-resolved two-photon excitation fluorescence (TPEF) images of drosophila egg-chambers.
| Original language | English |
|---|---|
| Title of host publication | Progress in Biomedical Optics and Imaging - Proceedings of SPIE |
| Editors | A. Periasamy, P.T.C. So |
| Pages | 35-43 |
| Number of pages | 9 |
| Volume | 5700 |
| DOIs | |
| Publication status | Published - 2005 |
| Event | Multiphoton Microscopy in the Biomedical Sciences V - San Jose, CA, United States Duration: Jan 23 2005 → Jan 25 2005 |
Other
| Other | Multiphoton Microscopy in the Biomedical Sciences V |
|---|---|
| Country | United States |
| City | San Jose, CA |
| Period | 1/23/05 → 1/25/05 |
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Keywords
- Laser-scanning
- Microscopy
- Nonlinear optics
- Two-photon excitation fluorescence
ASJC Scopus subject areas
- Engineering(all)
Cite this
Space multiplexed multiphoton microscopy by temporal focusing of ultrashort pulses. / Oron, Dan; Tal, Eran; Silberberg, Yaron.
Progress in Biomedical Optics and Imaging - Proceedings of SPIE. ed. / A. Periasamy; P.T.C. So. Vol. 5700 2005. p. 35-43 10.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - Space multiplexed multiphoton microscopy by temporal focusing of ultrashort pulses
AU - Oron, Dan
AU - Tal, Eran
AU - Silberberg, Yaron
PY - 2005
Y1 - 2005
N2 - The ability to perform optical sectioning is one of the great advantages of laser-scanning microscopy. This introduces, however, a number of difficulties due to the scanning process, such as lower frame rates due to the serial acquisition process. Here we show that by introducing spatiotemporal pulse shaping techniques to multiphoton microscopy it is possible to obtain full-frame depth resolved imaging completely without scanning. Our method relies on temporal focusing of the illumination pulse. The pulsed excitation field is compressed as it propagates through the sample, reaching its shortest duration at the focal plane, before stretching again beyond it. Combining temporal focusing with line-scanning microscopy results in an enhanced depth resolution, equivalent to that achieved by point scanning. Both the scanningless and the line-scanning techniques are applied to obtain depth-resolved two-photon excitation fluorescence (TPEF) images of drosophila egg-chambers.
AB - The ability to perform optical sectioning is one of the great advantages of laser-scanning microscopy. This introduces, however, a number of difficulties due to the scanning process, such as lower frame rates due to the serial acquisition process. Here we show that by introducing spatiotemporal pulse shaping techniques to multiphoton microscopy it is possible to obtain full-frame depth resolved imaging completely without scanning. Our method relies on temporal focusing of the illumination pulse. The pulsed excitation field is compressed as it propagates through the sample, reaching its shortest duration at the focal plane, before stretching again beyond it. Combining temporal focusing with line-scanning microscopy results in an enhanced depth resolution, equivalent to that achieved by point scanning. Both the scanningless and the line-scanning techniques are applied to obtain depth-resolved two-photon excitation fluorescence (TPEF) images of drosophila egg-chambers.
KW - Laser-scanning
KW - Microscopy
KW - Nonlinear optics
KW - Two-photon excitation fluorescence
UR - http://www.scopus.com/inward/record.url?scp=21844472252&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=21844472252&partnerID=8YFLogxK
U2 - 10.1117/12.597276
DO - 10.1117/12.597276
M3 - Conference contribution
AN - SCOPUS:21844472252
VL - 5700
SP - 35
EP - 43
BT - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
A2 - Periasamy, A.
A2 - So, P.T.C.
ER -