Space multiplexed multiphoton microscopy by temporal focusing of ultrashort pulses

Dan Oron, Eran Tal, Yaron Silberberg

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The ability to perform optical sectioning is one of the great advantages of laser-scanning microscopy. This introduces, however, a number of difficulties due to the scanning process, such as lower frame rates due to the serial acquisition process. Here we show that by introducing spatiotemporal pulse shaping techniques to multiphoton microscopy it is possible to obtain full-frame depth resolved imaging completely without scanning. Our method relies on temporal focusing of the illumination pulse. The pulsed excitation field is compressed as it propagates through the sample, reaching its shortest duration at the focal plane, before stretching again beyond it. Combining temporal focusing with line-scanning microscopy results in an enhanced depth resolution, equivalent to that achieved by point scanning. Both the scanningless and the line-scanning techniques are applied to obtain depth-resolved two-photon excitation fluorescence (TPEF) images of drosophila egg-chambers.

Original languageEnglish
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
EditorsA. Periasamy, P.T.C. So
Pages35-43
Number of pages9
Volume5700
DOIs
Publication statusPublished - 2005
EventMultiphoton Microscopy in the Biomedical Sciences V - San Jose, CA, United States
Duration: Jan 23 2005Jan 25 2005

Other

OtherMultiphoton Microscopy in the Biomedical Sciences V
CountryUnited States
CitySan Jose, CA
Period1/23/051/25/05

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Keywords

  • Laser-scanning
  • Microscopy
  • Nonlinear optics
  • Two-photon excitation fluorescence

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Oron, D., Tal, E., & Silberberg, Y. (2005). Space multiplexed multiphoton microscopy by temporal focusing of ultrashort pulses. In A. Periasamy, & P. T. C. So (Eds.), Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 5700, pp. 35-43). [10] https://doi.org/10.1117/12.597276