Structure and Raman spectrum of clavulanic acid in aqueous solution

Andrea Miani, Simone Raugei, Paolo Carloni, Marion S. Helfand

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8 Citations (Scopus)

Abstract

The calculation of the vibrational Raman spectrum of enzyme-bound β-lactamase inhibitors, may be of help to understand the mechanisms responsible for bacterial drug resistance. Here, we present a study of the solvation structure and the vibrational properties of clavulanate, an imporant β-lactamase inhibitor, in aqueous solution as obtained from full quantum and hybrid empirical/quantum molecular dynamics simulations at ambient conditions. The analysis of the vibrational density of states indicates that hybrid empirical/quantum mechanical simulations are able to properly describe the vibrational levels of clavulanate in solution. In addition, we propose a computationally efficient protocol to calculate the vibrational Raman effect for large solute molecules in water, which is able to faithfully reproduce the experimentally recorded clavulanate Raman spectrum and discloses the possibility to employ hybrid simulations to assign the experimental Raman spectra of inhibitors bound to β-lactamases.

Original languageEnglish
Pages (from-to)2621-2630
Number of pages10
JournalJournal of Physical Chemistry B
Volume111
Issue number10
DOIs
Publication statusPublished - Mar 15 2007

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ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Surfaces, Coatings and Films
  • Materials Chemistry

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