Structure of a photosynthetic reaction centre determined by serial femtosecond crystallography

Linda C. Johansson, David Arnlund, Gergely Katona, Thomas A. White, Anton Barty, Daniel P. Deponte, Robert L. Shoeman, Cecilia Wickstrand, Amit Sharma, Garth J. Williams, Andrew Aquila, Michael J. Bogan, Carl Caleman, Jan Davidsson, R. Bruce Doak, Matthias Frank, Raimund Fromme, Lorenzo Galli, Ingo Grotjohann, Mark S. Hunter & 25 others Stephan Kassemeyer, Richard A. Kirian, Christopher Kupitz, Mengning Liang, Lukas Lomb, Erik Malmerberg, Andrew V. Martin, Marc Messerschmidt, Karol Nass, Lars Redecke, M. Marvin Seibert, Jennie Sjöhamn, Jan Steinbrener, Francesco Stellato, Dingjie Wang, Weixaio Y. Wahlgren, Uwe Weierstall, Sebastian Westenhoff, Nadia A. Zatsepin, Sébastien Boutet, John C H Spence, Ilme Schlichting, Henry N. Chapman, Petra Fromme, Richard Neutze

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

Serial femtosecond crystallography is an X-ray free-electron-laser-based method with considerable potential to have an impact on challenging problems in structural biology. Here we present X-ray diffraction data recorded from microcrystals of the Blastochloris viridis photosynthetic reaction centre to 2.8 Å resolution and determine its serial femtosecond crystallography structure to 3.5 Å resolution. Although every microcrystal is exposed to a dose of 33 MGy, no signs of X-ray-induced radiation damage are visible in this integral membrane protein structure.

Original languageEnglish
Article number2911
JournalNature Communications
Volume4
DOIs
Publication statusPublished - Dec 19 2013

Fingerprint

Photosynthetic Reaction Center Complex Proteins
Crystallography
Microcrystals
X Ray Crystallography
X-Ray Diffraction
crystallography
Membrane Proteins
Lasers
microcrystals
X-Rays
Electrons
X ray lasers
Light
Free electron lasers
Radiation damage
Dosimetry
x rays
biology
radiation damage
free electron lasers

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Chemistry(all)
  • Physics and Astronomy(all)

Cite this

Johansson, L. C., Arnlund, D., Katona, G., White, T. A., Barty, A., Deponte, D. P., ... Neutze, R. (2013). Structure of a photosynthetic reaction centre determined by serial femtosecond crystallography. Nature Communications, 4, [2911]. https://doi.org/10.1038/ncomms3911

Structure of a photosynthetic reaction centre determined by serial femtosecond crystallography. / Johansson, Linda C.; Arnlund, David; Katona, Gergely; White, Thomas A.; Barty, Anton; Deponte, Daniel P.; Shoeman, Robert L.; Wickstrand, Cecilia; Sharma, Amit; Williams, Garth J.; Aquila, Andrew; Bogan, Michael J.; Caleman, Carl; Davidsson, Jan; Doak, R. Bruce; Frank, Matthias; Fromme, Raimund; Galli, Lorenzo; Grotjohann, Ingo; Hunter, Mark S.; Kassemeyer, Stephan; Kirian, Richard A.; Kupitz, Christopher; Liang, Mengning; Lomb, Lukas; Malmerberg, Erik; Martin, Andrew V.; Messerschmidt, Marc; Nass, Karol; Redecke, Lars; Seibert, M. Marvin; Sjöhamn, Jennie; Steinbrener, Jan; Stellato, Francesco; Wang, Dingjie; Wahlgren, Weixaio Y.; Weierstall, Uwe; Westenhoff, Sebastian; Zatsepin, Nadia A.; Boutet, Sébastien; Spence, John C H; Schlichting, Ilme; Chapman, Henry N.; Fromme, Petra; Neutze, Richard.

In: Nature Communications, Vol. 4, 2911, 19.12.2013.

Research output: Contribution to journalArticle

Johansson, LC, Arnlund, D, Katona, G, White, TA, Barty, A, Deponte, DP, Shoeman, RL, Wickstrand, C, Sharma, A, Williams, GJ, Aquila, A, Bogan, MJ, Caleman, C, Davidsson, J, Doak, RB, Frank, M, Fromme, R, Galli, L, Grotjohann, I, Hunter, MS, Kassemeyer, S, Kirian, RA, Kupitz, C, Liang, M, Lomb, L, Malmerberg, E, Martin, AV, Messerschmidt, M, Nass, K, Redecke, L, Seibert, MM, Sjöhamn, J, Steinbrener, J, Stellato, F, Wang, D, Wahlgren, WY, Weierstall, U, Westenhoff, S, Zatsepin, NA, Boutet, S, Spence, JCH, Schlichting, I, Chapman, HN, Fromme, P & Neutze, R 2013, 'Structure of a photosynthetic reaction centre determined by serial femtosecond crystallography', Nature Communications, vol. 4, 2911. https://doi.org/10.1038/ncomms3911
Johansson, Linda C. ; Arnlund, David ; Katona, Gergely ; White, Thomas A. ; Barty, Anton ; Deponte, Daniel P. ; Shoeman, Robert L. ; Wickstrand, Cecilia ; Sharma, Amit ; Williams, Garth J. ; Aquila, Andrew ; Bogan, Michael J. ; Caleman, Carl ; Davidsson, Jan ; Doak, R. Bruce ; Frank, Matthias ; Fromme, Raimund ; Galli, Lorenzo ; Grotjohann, Ingo ; Hunter, Mark S. ; Kassemeyer, Stephan ; Kirian, Richard A. ; Kupitz, Christopher ; Liang, Mengning ; Lomb, Lukas ; Malmerberg, Erik ; Martin, Andrew V. ; Messerschmidt, Marc ; Nass, Karol ; Redecke, Lars ; Seibert, M. Marvin ; Sjöhamn, Jennie ; Steinbrener, Jan ; Stellato, Francesco ; Wang, Dingjie ; Wahlgren, Weixaio Y. ; Weierstall, Uwe ; Westenhoff, Sebastian ; Zatsepin, Nadia A. ; Boutet, Sébastien ; Spence, John C H ; Schlichting, Ilme ; Chapman, Henry N. ; Fromme, Petra ; Neutze, Richard. / Structure of a photosynthetic reaction centre determined by serial femtosecond crystallography. In: Nature Communications. 2013 ; Vol. 4.
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