Structure of cytochrome c2 from Rhodospirillum centenum

A. Camara-Artigas, J. C. Williams, James Paul Allen

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Cytochrome c2 from the purple photosynthetic bacterium Rhodospirillum centenum has been crystallized by the sitting-drop vapour-diffusion method. The crystals belong to the orthorhombic space group P212121, with unit-cell parameters a = 29.7, b = 59.9, c = 65.4 Å, and diffract to a resolution limit of 1.7 Å. The Fe-atom position was determined from its anomalous scattering contribution and a molecular-replacement solution was calculated. The correctness of the solution was confirmed by parallel isomorphous replacement studies. The resulting model has a type I cytochrome fold with two features, an extended α-helix and a surface-charge distribution, that are distinctive to this protein. The implications of these structural features for the ability of the cytochrome to serve as an electron carrier are discussed.

Original languageEnglish
Pages (from-to)1498-1505
Number of pages8
JournalActa Crystallographica Section D: Biological Crystallography
Volume57
Issue number11
DOIs
Publication statusPublished - 2001

Fingerprint

Rhodospirillum centenum
Cytochromes c2
cytochromes
Cytochromes
Proteobacteria
Charge distribution
Surface charge
Bacteria
Vapors
Scattering
Electrons
Atoms
helices
charge distribution
bacteria
Crystals
vapors
proteins
Proteins
cells

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biophysics
  • Condensed Matter Physics
  • Structural Biology

Cite this

Structure of cytochrome c2 from Rhodospirillum centenum. / Camara-Artigas, A.; Williams, J. C.; Allen, James Paul.

In: Acta Crystallographica Section D: Biological Crystallography, Vol. 57, No. 11, 2001, p. 1498-1505.

Research output: Contribution to journalArticle

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