Structure of phenoxazinone synthase from Streptomyces antibioticus reveals a new type 2 copper center

Alex W. Smith, Ana Camara-Artigas, Meitian Wang, James Paul Allen, Wilson A. Francisco

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

The multicopper oxidase phenoxazinone synthase (PHS) catalyzes the penultimate step in the biosynthesis of the antibiotic actinomycin D by Streptomyces antibioticus. PHS exists in two oligomeric forms: a dimeric form and a hexameric form, with older actinomycin-producing cultures containing predominately the hexameric form. The structure of hexameric PHS has been determined using X-ray diffraction to a resolution limit of 2.30 Å and is found to contain several unexpected and distinctive features. The structure forms a hexameric ring that is centered on a pseudo 6-fold axis and has an outer diameter of 185 Å with a large central cavity that has a diameter of 50 Å. This hexameric structure is stabilized by a long loop connecting two domains; bound to this long loop is a fifth copper atom that is present as a type 2 copper. This copper atom is not present in any other multicopper oxidase, and its presence appears to stabilize the hexameric structure.

Original languageEnglish
Pages (from-to)4378-4387
Number of pages10
JournalBiochemistry
Volume45
Issue number14
DOIs
Publication statusPublished - Apr 11 2006

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Streptomyces antibioticus
Copper
Dactinomycin
Oxidoreductases
Atoms
Biosynthesis
X-Ray Diffraction
Anti-Bacterial Agents
X ray diffraction
phenoxazinone

ASJC Scopus subject areas

  • Biochemistry

Cite this

Structure of phenoxazinone synthase from Streptomyces antibioticus reveals a new type 2 copper center. / Smith, Alex W.; Camara-Artigas, Ana; Wang, Meitian; Allen, James Paul; Francisco, Wilson A.

In: Biochemistry, Vol. 45, No. 14, 11.04.2006, p. 4378-4387.

Research output: Contribution to journalArticle

Smith, Alex W. ; Camara-Artigas, Ana ; Wang, Meitian ; Allen, James Paul ; Francisco, Wilson A. / Structure of phenoxazinone synthase from Streptomyces antibioticus reveals a new type 2 copper center. In: Biochemistry. 2006 ; Vol. 45, No. 14. pp. 4378-4387.
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